PMID- 17345134
OWN - NLM
STAT- MEDLINE
DCOM- 20070912
LR  - 20220408
IS  - 0095-3628 (Print)
IS  - 0095-3628 (Linking)
VI  - 54
IP  - 2
DP  - 2007 Aug
TI  - Molecular characterization of sheep ruminal enrichments that detoxify 
      pyrrolizidine alkaloids by denaturing gradient gel electrophoresis and cloning.
PG  - 264-75
AB  - An enrichment of strictly anaerobic bacteria from ovine rumen fluid, which has 
      previously been named L4M2, is known to detoxify animal hepatotoxins from the 
      pyrrolizidine alkaloid family. These toxins are present in the tansy ragwort 
      plant (Senecio jacobaea). These plants have been described in livestock animals' 
      range forages in regions of the world such as the Northwest United States and 
      South Africa. The bacterial enrichment was characterized by molecular cloning 
      techniques and by the molecular fingerprinting technique of denaturing gradient 
      gel electrophoresis (DGGE). Phylogenetic analysis of the enrichment revealed that 
      the consortium is composed of no more than five putative bacterial species which 
      associated to the Anaerovibrio, Desulfovibrio, Megasphaera, Prevotella, and 
      Synergistes generas. These are all known to exist in the upper gastrointestinal 
      tract of ruminant animals. This work improved upon previous attempts to 
      characterize the consortium by obtaining nearly full-length ribosomal 16S rDNA 
      sequences through cloning. The DGGE results were directly compared to the cloning 
      data by polymerase chain reaction (PCR) amplifying eight phylogenetically 
      representative clones and analyzing them by DGGE. Direct DGGE analysis of the 
      enrichment displayed greater 16S diversity than the clone library used in this 
      study, suggesting that at least one of the organisms present in the enrichment 
      comprises less than 1% of the total cell population. These data will be used to 
      further refine the enrichment in hopes of future use as a probiotic, which could 
      be administered to animals challenged by the presence of tansy ragwort in their 
      forage.
FAU - Rattray, Rogan M
AU  - Rattray RM
AD  - Department of Microbiology, Oregon State University, 139 Oak Creek Building, 
      Corvallis, Oregon 97331, USA.
FAU - Craig, A Morrie
AU  - Craig AM
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070308
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Pyrrolizidine Alkaloids)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria, Anaerobic/*chemistry/genetics/metabolism
MH  - *Cloning, Molecular
MH  - DNA, Bacterial
MH  - DNA, Ribosomal/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Polymerase Chain Reaction
MH  - Pyrrolizidine Alkaloids/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rumen/metabolism/*microbiology
MH  - Senecio/chemistry/toxicity
MH  - Sheep
EDAT- 2007/03/09 09:00
MHDA- 2007/09/13 09:00
CRDT- 2007/03/09 09:00
PHST- 2006/12/13 00:00 [received]
PHST- 2006/12/15 00:00 [accepted]
PHST- 2006/12/13 00:00 [revised]
PHST- 2007/03/09 09:00 [pubmed]
PHST- 2007/09/13 09:00 [medline]
PHST- 2007/03/09 09:00 [entrez]
AID - 10.1007/s00248-006-9198-6 [doi]
PST - ppublish
SO  - Microb Ecol. 2007 Aug;54(2):264-75. doi: 10.1007/s00248-006-9198-6. Epub 2007 Mar 
      8.