PMID- 17346281
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20220224
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 121
IP  - 2
DP  - 2007 Jun
TI  - In vivo kinetics of human natural killer cells: the effects of ageing and acute 
      and chronic viral infection.
PG  - 258-65
AB  - Human natural killer (NK) cells form a circulating population in a state of 
      dynamic homeostasis. We investigated NK cell homeostasis by labelling dividing 
      cells in vivo using deuterium-enriched glucose in young and elderly healthy 
      subjects and patients with viral infection. Following a 24-hr intravenous 
      infusion of 6,6-D(2)-glucose, CD3(-) CD16(+) NK cells sorted from peripheral 
      blood mononuclear cells (PBMC) by fluorescence-activated cell sorter (FACS) were 
      analysed for DNA deuterium content by gas chromatography mass spectrometry to 
      yield minimum estimates for proliferation rate (p). In healthy young adults 
      (n=5), deuterium enrichment was maximal approximately 10 days after labelling, 
      consistent with postmitotic maturation preceding circulation. The mean (+/- 
      standard deviation) proliferation rate was 4 x 3 +/- 2 x 4%/day (equivalent to a 
      doubling time of 16 days) and the total production rate was 15 +/- (7 x 6) x 
      10(6) cells/l/day. Labelled cells disappeared from the circulation at a similar 
      rate [6 x 9 +/- 4 x 0%/day; half-life (T((1/2))) < 10 days]. Healthy elderly 
      subjects (n=8) had lower proliferation and production rates (P=2 x 5 +/- 1 x 
      0%/day and 7 x 3 +/- (3 x 7) x 10(6) cells/l/day, respectively; P=0 x 04). 
      Similar rates were seen in patients chronically infected with human T-cell 
      lymphotropic virus type I (HTLV-I) (P=3 x 2 +/- 1 x 9%/day). In acute infectious 
      mononucleosis (n=5), NK cell numbers were increased but kinetics were unaffected 
      (P=2 x 8 +/- 1 x 0%/day) a mean of 12 days after symptom onset. Human NK cells 
      have a turnover time in blood of about 2 weeks. Proliferation rates appear to 
      fall with ageing, remain unperturbed by chronic HTLV-I infection and normalize 
      rapidly following acute Epstein-Barr virus infection.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Centre for Infection, Division of Cellular & Molecular Medicine, St George's, 
      University of London, London, UK.
FAU - Wallace, Diana L
AU  - Wallace DL
FAU - de Lara, Catherine M
AU  - de Lara CM
FAU - Ghattas, Hala
AU  - Ghattas H
FAU - Asquith, Becca
AU  - Asquith B
FAU - Worth, Andrew
AU  - Worth A
FAU - Griffin, George E
AU  - Griffin GE
FAU - Taylor, Graham P
AU  - Taylor GP
FAU - Tough, David F
AU  - Tough DF
FAU - Beverley, Peter C L
AU  - Beverley PC
FAU - Macallan, Derek C
AU  - Macallan DC
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070307
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Aging/*immunology
MH  - Cell Proliferation
MH  - Chronic Disease
MH  - Female
MH  - HTLV-I Infections/immunology
MH  - Humans
MH  - Infectious Mononucleosis/immunology
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - Virus Diseases/*immunology
PMC - PMC2265941
EDAT- 2007/03/10 09:00
MHDA- 2007/06/19 09:00
PMCR- 2008/06/01
CRDT- 2007/03/10 09:00
PHST- 2007/03/10 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/03/10 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - IMM2573 [pii]
AID - 10.1111/j.1365-2567.2007.02573.x [doi]
PST - ppublish
SO  - Immunology. 2007 Jun;121(2):258-65. doi: 10.1111/j.1365-2567.2007.02573.x. Epub 
      2007 Mar 7.