PMID- 17346550
OWN - NLM
STAT- MEDLINE
DCOM- 20070503
LR  - 20071203
IS  - 1097-6868 (Electronic)
IS  - 0002-9378 (Linking)
VI  - 196
IP  - 3
DP  - 2007 Mar
TI  - Thrombin regulates monocyte chemoattractant protein-1 expression in human first 
      trimester and term decidual cells.
PG  - 268.e1-8
AB  - OBJECTIVE: Excess decidual macrophage infiltration has been linked to 
      preeclampsia and a failure of endovascular trophoblast invasion. Severe 
      preeclampsia with shallow placentation has also been linked to acquired and 
      inherited maternal thrombophilias and recurrent decidual hemorrhage, which 
      generates thrombin from decidual cell-expressed tissue factor. Therefore, the 
      current study evaluated whether thrombin affects monocyte chemoattractant 
      protein-1 (MCP-1) expression in stromal cells that are derived from cycling and 
      gestational endometrium. STUDY DESIGN: Stromal cells that are isolated from 
      cycling endometrium and first trimester and term decidua were grown to 
      confluence, treated for 7 days with 10(-8) mol/L estradiol (E2) + 10(-7) mol/L 
      medroxyprogesterone acetate (MPA), then switched to a serum-free medium that 
      contained the corresponding steroids +/- thrombin. MCP-1 protein release was 
      measured by enzyme-linked immunosorbent assay and Western blot; MCP-1 messenger 
      RNA levels were assessed by real-time quantitative reverse 
      transcription-polymerase chain reaction. RESULTS: Secreted MCP-1 levels were not 
      significantly different in stromal or decidual cell cultures that were incubated 
      with E2 or with E2 + MPA. Thrombin increased immunoreactive MCP-1 expression in a 
      dose-response fashion in first trimester and term decidual cells but not in 
      endometrial stromal cells. Thrombin-induced MCP-1 protein output was unaffected 
      by MPA but was abrogated by incubation with the thrombin inhibitor, hirudin. 
      Unexpectedly, thrombin-enhanced MCP-1 protein expression was unaccompanied by 
      corresponding changes in steady state MCP-1 messenger RNA levels, which suggests 
      its effects were posttranslational. CONCLUSION: MCP-1 protein expression is up 
      regulated by thrombin in decidual cells across gestation, but not in stromal 
      cells from predecidualized cycling endometrium.
FAU - Matta, Paul
AU  - Matta P
AD  - Department of Obstetrics, Gynecology & Reproductive Sciences, Yale University 
      School of Medicine, New Haven, CT 06520, USA.
FAU - Lockwood, Charles J
AU  - Lockwood CJ
FAU - Schatz, Frederick
AU  - Schatz F
FAU - Krikun, Graciela
AU  - Krikun G
FAU - Rahman, Mizanur
AU  - Rahman M
FAU - Buchwalder, Lynn
AU  - Buchwalder L
FAU - Norwitz, Errol R
AU  - Norwitz ER
LA  - eng
GR  - R01-HL070004-03/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Am J Obstet Gynecol
JT  - American journal of obstetrics and gynecology
JID - 0370476
RN  - 0 (Chemokine CCL2)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis
MH  - Decidua/*cytology/*metabolism
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Pregnancy Trimester, First
MH  - Term Birth
MH  - Thrombin/*physiology
EDAT- 2007/03/10 09:00
MHDA- 2007/05/04 09:00
CRDT- 2007/03/10 09:00
PHST- 2006/03/28 00:00 [received]
PHST- 2006/07/08 00:00 [revised]
PHST- 2006/09/14 00:00 [accepted]
PHST- 2007/03/10 09:00 [pubmed]
PHST- 2007/05/04 09:00 [medline]
PHST- 2007/03/10 09:00 [entrez]
AID - S0002-9378(06)01203-8 [pii]
AID - 10.1016/j.ajog.2006.09.008 [doi]
PST - ppublish
SO  - Am J Obstet Gynecol. 2007 Mar;196(3):268.e1-8. doi: 10.1016/j.ajog.2006.09.008.