PMID- 17346934
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20121115
IS  - 0923-1811 (Print)
IS  - 0923-1811 (Linking)
VI  - 46
IP  - 2
DP  - 2007 May
TI  - Increase of collagen synthesis by obovatol through stimulation of the TGF-beta 
      signaling and inhibition of matrix metalloproteinase in UVB-irradiated human 
      fibroblast.
PG  - 127-37
AB  - BACKGROUND: Alterations of the extracellular matrix (ECM) is critical in the 
      photo and age-damaged skin. Thus any compounds keep ECM can protected from photo 
      and aged-damaged skin. ECM is predominantly composed of type I and type III 
      collagens in the dermis. Transforming growth factor (TGF-beta)s play important 
      roles in cellular biosynthesis of extracellular matrix. Activator protein 1 
      (AP-1) and Smad are significant factors that mediate TGF-beta. OBJECTIVE: We have 
      investigated increasing effects of obovatol, a biphenolic compound isolated from 
      leaves of Magnolia obovata on the collagen synthesis through stimulation of the 
      TGF-beta signaling and inhibition of matrix metalloproteinase, thereby protect 
      against from UV damages via maintain of collagen in the UVB irradiated human 
      fibroblast cells. METHODS: The fibroblasts were pretreated with obovatol for 24h 
      and then the cells were irradiated with UVB. UVB-exposed cells were further 
      cultured for 24h. Type I procollagen, MMP-3, TGF-beta and Smad as well as 
      phosphorylation of MAPK family expression were determined by Western blot. The 
      activation of AP-1 was investigated using EMSA. The released type I procollagen 
      and TGF-beta into cell culture medium were determined by Western blot after 
      concentration of these proteins. RESULTS: The results showed that obovatol 
      stimulated type I procollagen, TGF-beta, and Smad expression and inhibited matrix 
      metalloproteinase-3 (MMP-3) in dose-dependent manner (1-5muM) in UVB-irradiated 
      human fibroblast cells. Obovatol also inhibited UVB-induced activation of AP-1 
      and MAP kinases. CONCLUSION: These results suggest that obovatol increases 
      collagen synthesis through stimulation of the TGF-beta signaling and inhibition 
      of matrix metalloproteinase in UVB-irradiated human fibroblast, thus obovatol 
      could be effective against photo-damaged skin.
FAU - Choi, Myoung Suk
AU  - Choi MS
AD  - College of Pharmacy, Chungbuk National University, 48, Gaesin-dong, Heungduk-gu, 
      Cheungju, Chungbuk 361-763, South Korea.
FAU - Yoo, Myung Sik
AU  - Yoo MS
FAU - Son, Dong Ju
AU  - Son DJ
FAU - Jung, Hai Young
AU  - Jung HY
FAU - Lee, Seung Ho
AU  - Lee SH
FAU - Jung, Jae Kyung
AU  - Jung JK
FAU - Lee, Beom Cheon
AU  - Lee BC
FAU - Yun, Yeo Pyo
AU  - Yun YP
FAU - Pyo, Hyeong Bae
AU  - Pyo HB
FAU - Hong, Jin Tae
AU  - Hong JT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070307
PL  - Netherlands
TA  - J Dermatol Sci
JT  - Journal of dermatological science
JID - 9011485
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Collagen Type I)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Phenyl Ethers)
RN  - 0 (Radiation-Protective Agents)
RN  - 0 (Smad Proteins)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Transforming Growth Factor beta)
RN  - 83864-78-2 (obovatol)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Biphenyl Compounds/isolation & purification/*pharmacology
MH  - Cells, Cultured
MH  - Collagen Type I/*biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/*drug effects/metabolism/radiation effects
MH  - Humans
MH  - Magnolia/chemistry
MH  - Matrix Metalloproteinase 3/metabolism
MH  - *Matrix Metalloproteinase Inhibitors
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Phenyl Ethers/isolation & purification/*pharmacology
MH  - Phosphorylation
MH  - Plant Leaves
MH  - Radiation-Protective Agents/isolation & purification/*pharmacology
MH  - Signal Transduction/*drug effects
MH  - Skin/drug effects/metabolism/radiation effects
MH  - Smad Proteins/metabolism
MH  - Transcription Factor AP-1/metabolism
MH  - Transforming Growth Factor beta/*metabolism
MH  - *Ultraviolet Rays
EDAT- 2007/03/10 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/03/10 09:00
PHST- 2006/06/23 00:00 [received]
PHST- 2006/12/13 00:00 [revised]
PHST- 2007/02/01 00:00 [accepted]
PHST- 2007/03/10 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/03/10 09:00 [entrez]
AID - S0923-1811(07)00054-0 [pii]
AID - 10.1016/j.jdermsci.2007.02.001 [doi]
PST - ppublish
SO  - J Dermatol Sci. 2007 May;46(2):127-37. doi: 10.1016/j.jdermsci.2007.02.001. Epub 
      2007 Mar 7.