PMID- 17349073
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20081121
IS  - 0007-1145 (Print)
IS  - 0007-1145 (Linking)
VI  - 97
IP  - 4
DP  - 2007 Apr
TI  - The APOB -516C/T polymorphism is associated with differences in insulin 
      sensitivity in healthy males during the consumption of diets with different fat 
      content.
PG  - 622-7
AB  - Several apo B polymorphic sites have been studied for their potential use as 
      markers for CHD in the population and for potential gene-diet interactions. Our 
      aim was to determine whether the presence of the -516C/T polymorphism in the APOB 
      gene promoter modifies insulin sensitivity to dietary fat. We studied fifty-nine 
      healthy volunteers (thirty men and twenty-nine women, thirty-six homozygotes for 
      the -516C allele (C/C) (nineteen males and seventeen females) and twenty-three 
      heterozygotes for the -516T allele (C/T) (eleven males and twelve females)). 
      Subjects consumed three diets during the feeding study, 4 weeks each: an SFA-rich 
      diet (38 % fat, 20 % SFA), followed by a carbohydrate (CHO)-rich diet (30 % fat, 
      55 % CHO) or a MUFA-rich diet (38 % fat, 22 % MUFA) following a randomised 
      cross-over design. For each diet, we investigated peripheral insulin sensitivity 
      with the insulin suppression test. Male carriers of the -516T allele showed a 
      significantly greater decrease in steady-state plasma glucose concentrations when 
      changing from the SFA-rich diet (9.18 (sd 1.35) mmol/l) to the MUFA (6.55 (sd 
      0.74) mmol/l) or the CHO (6.31 (sd 0.93) mmol/l) diets than did those who were 
      homozygous for the C allele (P = 0.040). Furthermore, C/T subjects presented 
      higher plasma NEFA values after consumption of the SFA diet compared with the 
      MUFA and CHO diets (P = 0.001). This effect was not observed in females (P = 
      0.908). Our findings show that male carriers of the -516T allele, C/T, have a 
      significant increase in insulin resistance after consumption of all diets, but 
      the difference is more exaggerated after the SFA diet compared with the MUFA- and 
      CHO-rich diets.
FAU - Perez-Martinez, Pablo
AU  - Perez-Martinez P
AD  - Unit of Lipids and Atherosclerosis, Hospital Universitario Reina Sofia, Avda. 
      Menendez Pidal, s/n. 14004, Cordoba, Spain. pablopermar@yahoo.es
FAU - Perez-Jimenez, Francisco
AU  - Perez-Jimenez F
FAU - Ordovas, Jose Maria
AU  - Ordovas JM
FAU - Moreno, Juan Antonio
AU  - Moreno JA
FAU - Moreno, Rafael
AU  - Moreno R
FAU - Fuentes, Francisco
AU  - Fuentes F
FAU - Ruano, Juan
AU  - Ruano J
FAU - Gomez, Purificacion
AU  - Gomez P
FAU - Marin, Carmen
AU  - Marin C
FAU - Lopez-Miranda, Jose
AU  - Lopez-Miranda J
LA  - eng
GR  - HL54776/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Apolipoproteins)
RN  - 0 (Apolipoproteins B)
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Fats)
RN  - 0 (Lipids)
SB  - IM
MH  - Adult
MH  - Apolipoproteins/blood
MH  - Apolipoproteins B/*genetics
MH  - Blood Glucose/metabolism
MH  - Cross-Over Studies
MH  - Diet
MH  - Dietary Fats/*administration & dosage
MH  - Female
MH  - Humans
MH  - Insulin Resistance/*genetics
MH  - Lipids/blood
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic/genetics
EDAT- 2007/03/14 09:00
MHDA- 2007/05/09 09:00
CRDT- 2007/03/14 09:00
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
AID - S0007114507659005 [pii]
AID - 10.1017/S0007114507659005 [doi]
PST - ppublish
SO  - Br J Nutr. 2007 Apr;97(4):622-7. doi: 10.1017/S0007114507659005.