PMID- 17349628
OWN - NLM
STAT- MEDLINE
DCOM- 20070606
LR  - 20181113
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 581
IP  - 7
DP  - 2007 Apr 3
TI  - Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in 
      rabbit hearts subjected to ischemia/reperfusion.
PG  - 1302-10
AB  - We have mapped the sites of ischemia/reperfusion-induced phosphorylation of 
      cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of 
      Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used 
      precursor ion scanning combined with neutral loss scanning and found that mature 
      CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 
      at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian 
      species. Molecular modeling suggests that phosphorylation sites of subunit I face 
      the inter membrane space while those of subunits IVi1 and Vb face the matrix 
      side.
FAU - Fang, Ji-Kang
AU  - Fang JK
AD  - Laboratory of Biochemistry, The Department of Animal Biology, School of 
      Veterinary Medicine, 3800 Spruce Street, University of Pennsylvania, 
      Philadelphia, PA 19104, United States.
FAU - Prabu, Subbuswamy K
AU  - Prabu SK
FAU - Sepuri, Naresh B
AU  - Sepuri NB
FAU - Raza, Haider
AU  - Raza H
FAU - Anandatheerthavarada, Hindupur K
AU  - Anandatheerthavarada HK
FAU - Galati, Domenico
AU  - Galati D
FAU - Spear, Joseph
AU  - Spear J
FAU - Avadhani, Narayan G
AU  - Avadhani NG
LA  - eng
GR  - R01 GM049683/GM/NIGMS NIH HHS/United States
GR  - GM-49683/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070228
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Protein Subunits)
RN  - 2ZD004190S (Threonine)
RN  - 452VLY9402 (Serine)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - TE7660XO1C (Glycine)
RN  - W12LH4V8V3 (tricine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromatography, Liquid
MH  - Electron Transport Complex IV/*chemistry/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glycine/analogs & derivatives/chemistry
MH  - Heart
MH  - Molecular Sequence Data
MH  - Myocardial Reperfusion Injury/*enzymology
MH  - Myocardium/*enzymology
MH  - Phosphorylation
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Protein Subunits/chemistry/metabolism
MH  - Rabbits
MH  - Serine/metabolism
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Threonine/metabolism
PMC - PMC1995084
MID - NIHMS20992
EDAT- 2007/03/14 09:00
MHDA- 2007/06/07 09:00
PMCR- 2007/09/28
CRDT- 2007/03/14 09:00
PHST- 2006/12/16 00:00 [received]
PHST- 2007/02/14 00:00 [revised]
PHST- 2007/02/19 00:00 [accepted]
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/06/07 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
PHST- 2007/09/28 00:00 [pmc-release]
AID - S0014-5793(07)00208-6 [pii]
AID - 10.1016/j.febslet.2007.02.042 [doi]
PST - ppublish
SO  - FEBS Lett. 2007 Apr 3;581(7):1302-10. doi: 10.1016/j.febslet.2007.02.042. Epub 
      2007 Feb 28.