PMID- 17350209 OWN - NLM STAT- MEDLINE DCOM- 20070821 LR - 20191210 IS - 0732-8893 (Print) IS - 0732-8893 (Linking) VI - 58 IP - 2 DP - 2007 Jun TI - Rapid identification of Fusobacterium nucleatum and Fusobacterium necrophorum by fluorescence in situ hybridization. PG - 255-9 AB - Identification of clinically relevant Fusobacterium spp. is hampered by their slow growth, their frequent occurrence in polymicrobial culture, and the low reliability of biochemical differentiation methods. A newly developed fluorescence in situ hybridization (FISH) assay allowed reliable and rapid identification of Fusobacterium necrophorum and Fusobacterium nucleatum from culture. Preliminary results show that the method offers the perspective for direct detection of these pathogens in blood cultures and abscess aspirates. FAU - Sigge, Anja AU - Sigge A AD - Department of Medical Microbiology and Hygiene, University Hospital of Ulm, D-89081 Ulm, Germany. anja.sigge@uniklinik-ulm.de FAU - Essig, Andreas AU - Essig A FAU - Wirths, Beate AU - Wirths B FAU - Fickweiler, Kristina AU - Fickweiler K FAU - Kaestner, Nicole AU - Kaestner N FAU - Wellinghausen, Nele AU - Wellinghausen N FAU - Poppert, Sven AU - Poppert S LA - eng PT - Evaluation Study PT - Journal Article DEP - 20070309 PL - United States TA - Diagn Microbiol Infect Dis JT - Diagnostic microbiology and infectious disease JID - 8305899 SB - IM MH - Fusobacterium Infections/*diagnosis/genetics MH - Fusobacterium necrophorum/genetics/*isolation & purification MH - Fusobacterium nucleatum/genetics/*isolation & purification MH - Humans MH - In Situ Hybridization, Fluorescence/*methods MH - Sensitivity and Specificity EDAT- 2007/03/14 09:00 MHDA- 2007/08/22 09:00 CRDT- 2007/03/14 09:00 PHST- 2006/07/03 00:00 [received] PHST- 2006/12/06 00:00 [revised] PHST- 2007/01/08 00:00 [accepted] PHST- 2007/03/14 09:00 [pubmed] PHST- 2007/08/22 09:00 [medline] PHST- 2007/03/14 09:00 [entrez] AID - S0732-8893(07)00008-9 [pii] AID - 10.1016/j.diagmicrobio.2007.01.001 [doi] PST - ppublish SO - Diagn Microbiol Infect Dis. 2007 Jun;58(2):255-9. doi: 10.1016/j.diagmicrobio.2007.01.001. Epub 2007 Mar 9.