PMID- 17350464
OWN - NLM
STAT- MEDLINE
DCOM- 20070411
LR  - 20070312
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 174
IP  - 1
DP  - 2007 Apr 1
TI  - Characterization of trisomy 8 in pediatric undifferentiated sarcomas using 
      advanced molecular cytogenetic techniques.
PG  - 35-41
AB  - Pediatric undifferentiated soft tissue sarcomas (USTS) are a rare group of 
      neoplasms that are unclassifiable despite the application of immunohistochemical, 
      cytogenetic, and molecular techniques. To date, there is a dearth of studies 
      looking at the cytogenetic and molecular genetic alterations in such tumors. 
      Trisomy 8, a frequent molecular alteration in neoplasia, is seen in several soft 
      tissue sarcomas, including Ewing sarcoma/primitive neuroectodermal tumor 
      (ES/PNET), synovial sarcoma, and leiomyosarcoma. Because USTS share several 
      clinicobiological features with the aforementioned tumors, the occurrence of 
      alterations in chromosome 8 was studied in 11 pediatric USTS using a combination 
      of interphase fluorescence in situ hybridization (FISH), spectral karyotyping 
      (SKY), and genomic profiling with oligonucleotide array comparative genomic 
      hybridization (aCGH). The copy number status of MYC was also assessed on the same 
      tumors using dual-color FISH, with the aim of delineating the degree and 
      intratumoral distribution of MYC amplification in this tumor. A near-uniform 
      presence of an increase in MYC copy number was observed, along with an increase 
      in chromosome 8 copy number in all the tumors. SKY and aCGH analysis of tumors 
      exhibiting trisomy 8 confirmed the numerical imbalances. The occurrence of 
      trisomy 8 in a subset of pediatric USTS confirms a shared genomic alteration with 
      several other soft tissue sarcomas. Further studies are required to determine the 
      clinical implications of such a finding.
FAU - Selvarajah, Shamini
AU  - Selvarajah S
AD  - Department of Pathology and Laboratory Medicine, The Hospital for Sick Children, 
      555 University Avenue, Room 3-206, Toronto, Ontario M5G 1X8, Canada.
FAU - Yoshimoto, Maisa
AU  - Yoshimoto M
FAU - Prasad, Mona
AU  - Prasad M
FAU - Shago, Mary
AU  - Shago M
FAU - Squire, Jeremy A
AU  - Squire JA
FAU - Zielenska, Maria
AU  - Zielenska M
FAU - Somers, Gino R
AU  - Somers GR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - *Cytogenetic Analysis
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - Sarcoma/*genetics
MH  - Spectral Karyotyping
MH  - Trisomy/*genetics
EDAT- 2007/03/14 09:00
MHDA- 2007/04/12 09:00
CRDT- 2007/03/14 09:00
PHST- 2006/09/27 00:00 [received]
PHST- 2006/11/13 00:00 [revised]
PHST- 2006/11/21 00:00 [accepted]
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/04/12 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
AID - S0165-4608(06)00774-6 [pii]
AID - 10.1016/j.cancergencyto.2006.11.011 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Apr 1;174(1):35-41. doi: 
      10.1016/j.cancergencyto.2006.11.011.