PMID- 17350480
OWN - NLM
STAT- MEDLINE
DCOM- 20070605
LR  - 20071115
IS  - 0188-4409 (Print)
IS  - 0188-4409 (Linking)
VI  - 38
IP  - 3
DP  - 2007 Apr
TI  - Cytogenetic profile of childhood acute lymphoblastic leukemia in Oman.
PG  - 305-12
AB  - BACKGROUND: Chromosomal abnormalities have important diagnostic and prognostic 
      significance in acute lymphoblastic leukemia (ALL). The purpose of this study was 
      to define and classify the frequency and type of chromosomal abnormalities among 
      newly diagnosed children with ALL and compare the results with those reported 
      from other geographical regions of the world. METHODS: Bone marrow chromosomal 
      studies with GTG banding were performed in untreated ALL pediatric patients aged 
      from 7 days to 14 years. RESULTS: Among Omani children examined with ALL, 47 
      (81%) patients yielded results, with 26 (55.3%) showing an abnormal karyotype [10 
      (21.3%) pseudodiploid, 2 (4.3%) hypodiploid and 14 (29.7%) hyperdiploidy] and 21 
      (44.6%) had normal diploidy. Structural abnormalities were observed in 16 (34%), 
      of which 11 (23.4%) cases were translocations, the most frequent being t(9;22) 
      observed in three (6.4%) of our patients. Uncommon translocations such as 
      t(9;15)(p11;q10), t(3;6)(p12;q11), t(1;6)(?31;?q23), t(1;19)(q12;q12), 
      der(18)t(12;18)(q11;p11), and other structural aberrations add(2)(q22), 
      add(6)(q16), add(18)(q22), add(14)(q32) along with deletions del(10)(q22), 
      del(12)(p11), del(12)(p12), del(18)(q11) were also observed. CONCLUSIONS: The 
      study showed a good correlation and concordance between the ploidy distribution 
      by cytogenetics and flow cytometry. The patterns of chromosomal anomalies in our 
      patients showed some variations in the frequency of aberrations reported. It is 
      therefore necessary that newer techniques like fluorescence in situ hybridization 
      (FISH) along with reverse transcriptase polymerase chain reaction (RT-PCR) and 
      spectral karyotyping will help us identify chromosomal aberrations not detected 
      by conventional cytogenetic methods in the near future. To our knowledge, this is 
      the first report from the Middle East of a cytogenetic study on childhood ALL.
FAU - Udayakumar, Achandira Muthappa
AU  - Udayakumar AM
AD  - Department of Genetics, College of Medicine and Health Sciences, Sultan Qaboos 
      University, and Hospital, Muscat, Sultanate of Oman. udaya@squ.edu.om
FAU - Bashir, Wafa Ahmed
AU  - Bashir WA
FAU - Pathare, Anil Vasant
AU  - Pathare AV
FAU - Wali, Yasser Ahmed
AU  - Wali YA
FAU - Zacharia, Mathew
AU  - Zacharia M
FAU - Khan, Ashfaq Ahmed
AU  - Khan AA
FAU - Soliman, Heba
AU  - Soliman H
FAU - Al-Lamki, Zakia
AU  - Al-Lamki Z
FAU - Raeburn, John Alexander
AU  - Raeburn JA
LA  - eng
PT  - Journal Article
DEP - 20070122
PL  - United States
TA  - Arch Med Res
JT  - Archives of medical research
JID - 9312706
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Cytogenetics
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Oman
MH  - Ploidies
MH  - Precursor Cell Lymphoblastic 
      Leukemia-Lymphoma/diagnosis/*genetics/physiopathology
EDAT- 2007/03/14 09:00
MHDA- 2007/06/06 09:00
CRDT- 2007/03/14 09:00
PHST- 2006/07/19 00:00 [received]
PHST- 2006/10/12 00:00 [accepted]
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/06/06 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
AID - S0188-4409(06)00374-2 [pii]
AID - 10.1016/j.arcmed.2006.10.006 [doi]
PST - ppublish
SO  - Arch Med Res. 2007 Apr;38(3):305-12. doi: 10.1016/j.arcmed.2006.10.006. Epub 2007 
      Jan 22.