PMID- 17350752
OWN - NLM
STAT- MEDLINE
DCOM- 20070629
LR  - 20131121
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 270
IP  - 1-2
DP  - 2007 May 30
TI  - The interaction and cellular localization of HSP27 and ERbeta are modulated by 
      17beta-estradiol and HSP27 phosphorylation.
PG  - 33-42
AB  - Recently, we identified heat shock protein 27 (HSP27) as an estrogen 
      receptor-beta (ERbeta) associated protein that acts as a co-repressor of estrogen 
      signaling and serves as a biomarker of atherosclerosis. In this study, we sought 
      to further characterize the subcellular interaction of HSP27 and ERbeta, as well 
      as explore the factors that may modulate this interaction. In vitro we determined 
      that phosphorylated HSP27 is retained in the cytoplasm after treatment with 
      17beta-estradiol and to a lesser extent with heat shock. Under all experimental 
      conditions ERbeta was found to be slightly more abundant in the cytoplasm 
      compared to the nucleus. HSP27 and ERbeta associate in both the cytoplasm and 
      nucleus, however, co-localization studies reveal that in the presence of 
      17beta-estradiol, a significant portion of this interaction occurs outside of the 
      nucleus. These data highlight an extranuclear interaction between ERbeta and 
      HSP27 that may be of potential importance in modulating estrogen signaling.
FAU - Al-Madhoun, Ashraf Said
AU  - Al-Madhoun AS
AD  - University of Ottawa Heart Institute, 40 Ruskin St., Ottawa, Ontario K1Y 4W7, 
      Canada.
FAU - Chen, Yong-Xiang
AU  - Chen YX
FAU - Haidari, Leila
AU  - Haidari L
FAU - Rayner, Katey
AU  - Rayner K
FAU - Gerthoffer, William
AU  - Gerthoffer W
FAU - McBride, Heidi
AU  - McBride H
FAU - O'Brien, Edward R
AU  - O'Brien ER
LA  - eng
GR  - HL077726/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070213
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor beta/*metabolism
MH  - Fluorescence
MH  - HeLa Cells
MH  - Heat-Shock Proteins/chemistry/*metabolism
MH  - Heat-Shock Response/drug effects
MH  - Humans
MH  - Phosphorylation/drug effects
MH  - Protein Binding/drug effects
MH  - Protein Structure, Tertiary
MH  - Protein Transport/drug effects
MH  - Recombinant Fusion Proteins/metabolism
MH  - Subcellular Fractions/drug effects
EDAT- 2007/03/14 09:00
MHDA- 2007/06/30 09:00
CRDT- 2007/03/14 09:00
PHST- 2006/09/10 00:00 [received]
PHST- 2007/02/04 00:00 [revised]
PHST- 2007/02/06 00:00 [accepted]
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/06/30 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
AID - S0303-7207(07)00078-0 [pii]
AID - 10.1016/j.mce.2007.02.002 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2007 May 30;270(1-2):33-42. doi: 10.1016/j.mce.2007.02.002. 
      Epub 2007 Feb 13.