PMID- 17353092 OWN - NLM STAT- MEDLINE DCOM- 20070725 LR - 20161124 IS - 0304-3940 (Print) IS - 0304-3940 (Linking) VI - 417 IP - 2 DP - 2007 May 1 TI - Neurotrophin gene expression in rat brain under the action of Semax, an analogue of ACTH 4-10. PG - 201-5 AB - The heptapeptide Semax, an analogue of the N-terminal adrenocorticotropic hormone fragment (4-10) (ACTH(4-10)), has been shown to exert a number of neuroprotective effects. There are some investigations that connected these effects with the increase of neurotrophin gene expression under the peptide drug application in neuron cell cultures [M.I. Shadrina, O.V. Dolotov, I.A. Grivennikov, P.A. Slominsky, L.A. Andreeva, L.S. Inozemtseva, S.A. Limborska, N.F. Myasoedov, Rapid induction of neurotrophin mRNAs in rat glial cell cultures by Semax, an adrenocorticotropic hormone analogue, Neurosci. Lett. 308 (2001) (2) 115-118]. In this work, we examined the action of Semax on rapid changes of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) gene expression in vivo. Male Wistar rats were treated for 1h with Semax (50 microg/kg, single intranasal application) and neurotrophin gene expression in rat brain was analyzed by real-time polymerase chain reaction (PCR). It was revealed that an intranasal application of Semax increased the expression of both neurotrophin genes in rat hippocampus. Bdnf gene expression also increased in the brainstem and cerebellum. Ngf gene expression decreased in rat frontal cortex. Thus, Semax induces rapid, gene- and region-specific changes in neurotrophin gene expression in normal rat brain. FAU - Agapova, T Y AU - Agapova TY AD - Laboratory of Molecular Genetics of Hereditary Diseases, Department of Molecular Basis of Human Genetics, Institute of Molecular Genetics RAS, 2 Kurchatov Sq., Moscow 123182, Russia. phoenixa@yandex.ru FAU - Agniullin, Y V AU - Agniullin YV FAU - Shadrina, M I AU - Shadrina MI FAU - Shram, S I AU - Shram SI FAU - Slominsky, P A AU - Slominsky PA FAU - Lymborska, S A AU - Lymborska SA FAU - Myasoedov, N F AU - Myasoedov NF LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070220 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Nerve Growth Factors) RN - 0 (Neuroprotective Agents) RN - 0 (Peptide Fragments) RN - 0 (RNA, Messenger) RN - 80714-61-0 (ACTH (4-7), Pro-Gly-Pro-) RN - 9002-60-2 (Adrenocorticotropic Hormone) RN - 9061-61-4 (Nerve Growth Factor) RN - J48AU3I790 (ACTH (4-10)) SB - IM MH - Adrenocorticotropic Hormone/agonists/*analogs & derivatives/metabolism/pharmacology MH - Animals MH - Brain/anatomy & histology/*drug effects/metabolism MH - Brain-Derived Neurotrophic Factor/genetics MH - Down-Regulation/drug effects/physiology MH - Gene Expression/*drug effects/physiology MH - Male MH - Nerve Growth Factor/genetics MH - Nerve Growth Factors/*genetics MH - Neuroprotective Agents/*pharmacology MH - Peptide Fragments/*agonists/metabolism/*pharmacology MH - RNA, Messenger/drug effects/metabolism MH - Rats MH - Rats, Wistar MH - Up-Regulation/drug effects/physiology EDAT- 2007/03/14 09:00 MHDA- 2007/07/26 09:00 CRDT- 2007/03/14 09:00 PHST- 2006/12/13 00:00 [received] PHST- 2007/02/01 00:00 [revised] PHST- 2007/02/14 00:00 [accepted] PHST- 2007/03/14 09:00 [pubmed] PHST- 2007/07/26 09:00 [medline] PHST- 2007/03/14 09:00 [entrez] AID - S0304-3940(07)00210-8 [pii] AID - 10.1016/j.neulet.2007.02.042 [doi] PST - ppublish SO - Neurosci Lett. 2007 May 1;417(2):201-5. doi: 10.1016/j.neulet.2007.02.042. Epub 2007 Feb 20.