PMID- 17354256
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20201209
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 212
IP  - 1
DP  - 2007 May
TI  - Selective upregulation and amplification of the lysyl oxidase like-4 (LOXL4) gene 
      in head and neck squamous cell carcinoma.
PG  - 74-82
AB  - Members of the lysyl oxidase family (LOX) are copper and lysyl-tyrosine quinone 
      cofactor-containing amine oxidases that are important for the assembly and 
      maintenance of components of the extracellular matrix. Our previous results 
      demonstrated that a novel member, LOXL4, is overexpressed in head and neck 
      squamous cell carcinoma (HNSCC) compared to normal squamous epithelium. Results 
      of the current study showed overexpression of the LOXL4 transcript in 74% (46 of 
      62) of invasive HNSCC tumours and 90% of both primary and metastatic HNSCC cell 
      lines. Significant correlation was found between LOXL4 expression and local lymph 
      node metastases versus primary tumour types (p<0.01) and higher tumour stages 
      (p<0.01). Immunocytochemistry demonstrated cellular overexpression of the LOXL4 
      protein that correlated with the increased mRNA transcription in HNSCC cells. 
      HNSCC cell lines displayed in significant subset of nuclei increased copies of 
      the LOX4 gene locus on chromosome 10q24, demonstrated by fluorescence in situ 
      hybridization (FISH). Extensive metaphase cytogenetic analysis was performed on 
      UTSCC19A cells, identifying an isochromosome i(10)(q10). Taken together, these 
      results highlight LOXL4 expression as a distinctive trait and suggest a 
      functional role for LOXL4 in the molecular pathogenesis of invasive head and neck 
      carcinomas.
CI  - Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published 
      by John Wiley & Sons, Ltd.
FAU - Gorogh, T
AU  - Gorogh T
AD  - Division of Experimental Oncology, Department of Otorhinolaryngology, Head and 
      Neck Surgery, University of Kiel, Germany. gorogh@hno.uni-kiel.de
FAU - Weise, J B
AU  - Weise JB
FAU - Holtmeier, C
AU  - Holtmeier C
FAU - Rudolph, P
AU  - Rudolph P
FAU - Hedderich, J
AU  - Hedderich J
FAU - Gottschlich, S
AU  - Gottschlich S
FAU - Hoffmann, M
AU  - Hoffmann M
FAU - Ambrosch, P
AU  - Ambrosch P
FAU - Csiszar, K
AU  - Csiszar K
LA  - eng
GR  - AR47662/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.- (LOXL4 protein, human)
RN  - EC 1.4.3.13 (Protein-Lysine 6-Oxidase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Amino Acid Oxidoreductases/genetics/*metabolism
MH  - Blotting, Northern/methods
MH  - Carcinoma, Squamous Cell/*enzymology/genetics/secondary
MH  - Case-Control Studies
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 10
MH  - Cytogenetic Analysis
MH  - Enzyme Activation
MH  - Extracellular Matrix/enzymology
MH  - Female
MH  - Head and Neck Neoplasms/*enzymology/genetics
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Protein-Lysine 6-Oxidase
MH  - *Up-Regulation
EDAT- 2007/03/14 09:00
MHDA- 2007/06/19 09:00
CRDT- 2007/03/14 09:00
PHST- 2007/03/14 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/03/14 09:00 [entrez]
AID - 10.1002/path.2137 [doi]
PST - ppublish
SO  - J Pathol. 2007 May;212(1):74-82. doi: 10.1002/path.2137.