PMID- 17356257
OWN - NLM
STAT- MEDLINE
DCOM- 20070503
LR  - 20171116
IS  - 1421-9697 (Electronic)
IS  - 0250-6807 (Linking)
VI  - 51
IP  - 1
DP  - 2007
TI  - Effects of bioactive peptide, valyl-prolyl-proline (VPP), and lactobacillus 
      helveticus fermented milk containing VPP on bone loss in ovariectomized rats.
PG  - 65-74
AB  - BACKGROUND: Valyl-prolyl-proline (VPP), a bioactive peptide formed during the 
      fermentation with Lactobacillus helveticus LBK-16H (L. helveticus), has been 
      shown to increase bone formation in vitro. The aim of the study was to determine 
      whether VPP and L. helveticus fermented milk prevent bone loss in ovariectomized 
      (OVX) rats. METHODS: During the 12-week intervention study, the OVX rats received 
      VPP in water or L. helveticus fermented milk, containing VPP. Sham-operated rats 
      receiving water acted as controls. The trabecular and cortical bone mineral 
      density were determined by peripheral quantitative computed tomography before the 
      operation and at 4 and 12 weeks. The mechanical testing and ash weight analysis 
      as well as the static and dynamic histomorphometrical parameters were assessed at 
      the end of the intervention. RESULTS: VPP given in water showed no clear effect 
      on bone loss. L. helveticus fermented milk prevented bone loss by decreasing bone 
      turnover and increasing the bone mineral density. Ovariectomy caused a 57% loss 
      in the trabecular bone, which was attenuated by 16% in the L. helveticus group. 
      CONCLUSIONS: VPP peptide did not prevent ovariectomy-induced bone loss, which 
      could be due to the poor bioavailability of VPP from water solution. L. 
      helveticus fermented milk prevented bone loss, whether this is due to the VPP 
      peptide cannot be concluded.
CI  - Copyright 2007 S. Karger AG, Basel.
FAU - Narva, Mirkka
AU  - Narva M
AD  - Institute of Biomedicine, Pharmacology, University of Helsinki, Helsinki, 
      Finland.
FAU - Rissanen, Jukka
AU  - Rissanen J
FAU - Halleen, Jussi
AU  - Halleen J
FAU - Vapaatalo, Heikki
AU  - Vapaatalo H
FAU - Vaananen, Kalervo
AU  - Vaananen K
FAU - Korpela, Riitta
AU  - Korpela R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070315
PL  - Switzerland
TA  - Ann Nutr Metab
JT  - Annals of nutrition & metabolism
JID - 8105511
RN  - 0 (Oligopeptides)
RN  - 2U1R84870F (valyl-prolyl-proline)
SB  - IM
MH  - Animals
MH  - Bone Density/*drug effects
MH  - *Cultured Milk Products
MH  - Female
MH  - Lactobacillus helveticus
MH  - Oligopeptides/*pharmacology
MH  - Osteoporosis/etiology/*prevention & control
MH  - Ovariectomy/adverse effects
MH  - Probiotics/*pharmacology
MH  - Rats
EDAT- 2007/03/16 09:00
MHDA- 2007/05/04 09:00
CRDT- 2007/03/16 09:00
PHST- 2005/12/16 00:00 [received]
PHST- 2006/06/01 00:00 [accepted]
PHST- 2007/03/16 09:00 [pubmed]
PHST- 2007/05/04 09:00 [medline]
PHST- 2007/03/16 09:00 [entrez]
AID - 000100823 [pii]
AID - 10.1159/000100823 [doi]
PST - ppublish
SO  - Ann Nutr Metab. 2007;51(1):65-74. doi: 10.1159/000100823. Epub 2007 Mar 15.