PMID- 17356705
OWN - NLM
STAT- MEDLINE
DCOM- 20070412
LR  - 20190608
IS  - 1476-5586 (Electronic)
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Linking)
VI  - 9
IP  - 2
DP  - 2007 Feb
TI  - Distribution of menin-occupied regions in chromatin specifies a broad role of 
      menin in transcriptional regulation.
PG  - 101-7
AB  - Menin is the protein product of the MEN1 tumor-suppressor gene; one allele of 
      MEN1 is inactivated in the germ line of patients with "multiple endocrine 
      neoplasia type 1" (MEN1) cancer syndrome. Menin interacts with several proteins 
      involved in transcriptional regulation. RNA expression analyses have identified 
      several menin-regulated genes that could represent proximal or distal interaction 
      sites for menin. This report presents a substantial and unbiased sampling of 
      menin-occupied chromatin regions using Serial Analysis of Chromatin Occupancy; 
      this method combines chromatin immuno-precipitation with Serial Analysis of Gene 
      Expression. Hundreds of menin-occupied genomic sites were identified in promoter 
      regions (32% of menin-occupied loci), near the 3' end of genes (14%), or inside 
      genes (21%), extending other data about menin recruitments to many sites of 
      transcriptional activity. A large number of menin-occupied sites (33%) were 
      located outside known gene regions. Additional annotation of the human genome 
      could help in identifying genes at these loci, or these might be gene-free 
      regions of the genome where menin occupancy could play some structural or 
      regulatory role. Menin occupancy at many intragenic positions distant from the 
      core promoter reveals an unexpected type of menin target region at many loci in 
      the genome. These unbiased data also suggest that menin could play a broad role 
      in transcriptional regulation.
FAU - Agarwal, Sunita K
AU  - Agarwal SK
AD  - National Institute of Diabetes and Digestive and Kidney Diseases, National 
      Institutes of Health, Bethesda, MD 20892-1802, USA. sunitaa@mail.nih.gov
FAU - Impey, Soren
AU  - Impey S
FAU - McWeeney, Shannon
AU  - McWeeney S
FAU - Scacheri, Peter C
AU  - Scacheri PC
FAU - Collins, Francis S
AU  - Collins FS
FAU - Goodman, Richard H
AU  - Goodman RH
FAU - Spiegel, Allen M
AU  - Spiegel AM
FAU - Marx, Stephen J
AU  - Marx SJ
LA  - eng
GR  - R37 DK045423/DK/NIDDK NIH HHS/United States
GR  - DK45423/DK/NIDDK NIH HHS/United States
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Chromatin)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - 3' Untranslated Regions/chemistry/genetics
MH  - 5' Untranslated Regions/chemistry/genetics
MH  - Chromatin/*genetics
MH  - DNA/genetics/*metabolism
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation/genetics/*physiology
MH  - Gene Library
MH  - HeLa Cells
MH  - Humans
MH  - Immunoprecipitation
MH  - Multiple Endocrine Neoplasia Type 1/genetics
MH  - Plasmids/genetics
MH  - Polymerase Chain Reaction
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/analysis/*physiology
MH  - Transcription, Genetic/genetics/*physiology
PMC - PMC1813935
EDAT- 2007/03/16 09:00
MHDA- 2007/04/14 09:00
PMCR- 2007/02/01
CRDT- 2007/03/16 09:00
PHST- 2006/11/03 00:00 [received]
PHST- 2006/12/27 00:00 [revised]
PHST- 2006/12/29 00:00 [accepted]
PHST- 2007/03/16 09:00 [pubmed]
PHST- 2007/04/14 09:00 [medline]
PHST- 2007/03/16 09:00 [entrez]
PHST- 2007/02/01 00:00 [pmc-release]
AID - 06706 [pii]
AID - 10.1593/neo.06706 [doi]
PST - ppublish
SO  - Neoplasia. 2007 Feb;9(2):101-7. doi: 10.1593/neo.06706.