PMID- 17356792
OWN - NLM
STAT- MEDLINE
DCOM- 20070904
LR  - 20181113
IS  - 0301-486X (Print)
IS  - 0301-486X (Linking)
VI  - 163
IP  - 3
DP  - 2007 Mar
TI  - Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of 
      Paracoccidioides brasiliensis.
PG  - 117-28
AB  - Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic 
      fungus, Paracoccidioides brasiliensis. The present study investigated the 
      protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction 
      (approximately 380 kDa) in experimental murine PCM. In the first step, lymphocyte 
      proliferation and production of IFNgamma (but not IL-4) were observed in "in 
      vitro" spleen cells (from female BALB/c mice infected (i.v.) with P. 
      brasiliensis) that were stimulated with hMM fractions. In the second step, female 
      BALB/c mice were previously immunized (s.c.) with hMM fraction (25 microg/protein 
      = F-25 and 50 microg/protein = F-50), and the colony-forming units (CFU) of the 
      lung and spleen, the histopathological characteristics of the granulomatous 
      lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were 
      analyzed at 28 and 56 days after infection. The lung and liver CFU were lower in 
      mice previously immunized with the hMM fraction (P < 0.05). The granulomatous 
      lesions revealed a greater degree of compaction and organization, with no 
      dissemination of the fungus to other organs. Lower soluble antigen levels (P < 
      0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized 
      groups. The results for CFU, histopathology and antigenemia suggest that the hMM 
      fraction has a protective effect in experimental paracoccidioidomycosis in BALB/c 
      mice.
FAU - Pavanelli, Wander R
AU  - Pavanelli WR
AD  - Fundacao Araucaria, Curitiba, PR, Brazil.
FAU - Kaminami, Mari S
AU  - Kaminami MS
FAU - Geres, Juliana R
AU  - Geres JR
FAU - Sano, Ayako
AU  - Sano A
FAU - Ono, Mario A
AU  - Ono MA
FAU - Camargo, I C C
AU  - Camargo IC
FAU - Itano, Eiko N
AU  - Itano EN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070314
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/blood
MH  - Antigens, Fungal/blood/*immunology/isolation & purification
MH  - Chromatography
MH  - Colony Count, Microbial
MH  - Cytokines/immunology
MH  - Female
MH  - Fungal Proteins/blood
MH  - Glycoproteins/blood
MH  - Granuloma/pathology
MH  - Immunization
MH  - Immunoglobulin G/blood
MH  - Liver/microbiology/pathology
MH  - Lung/microbiology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Paracoccidioides/growth & development/*immunology
MH  - Paracoccidioidomycosis/*immunology/microbiology/pathology
MH  - Spleen/cytology/pathology
EDAT- 2007/03/16 09:00
MHDA- 2007/09/05 09:00
CRDT- 2007/03/16 09:00
PHST- 2006/10/30 00:00 [received]
PHST- 2007/01/29 00:00 [accepted]
PHST- 2007/03/16 09:00 [pubmed]
PHST- 2007/09/05 09:00 [medline]
PHST- 2007/03/16 09:00 [entrez]
AID - 10.1007/s11046-007-0095-4 [doi]
PST - ppublish
SO  - Mycopathologia. 2007 Mar;163(3):117-28. doi: 10.1007/s11046-007-0095-4. Epub 2007 
      Mar 14.