PMID- 1735957
OWN - NLM
STAT- MEDLINE
DCOM- 19920309
LR  - 20071114
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 66
IP  - 2
DP  - 1992 Feb
TI  - Biological characteristics of primary cultures of human gallbladder epithelial 
      cells.
PG  - 243-50
AB  - Epithelial cells of the gallbladder have potential to represent an important 
      model for studies of ductal epithelial in normal and pathological states. We 
      therefore initiated studies to establish human gallbladder epithelial cells 
      (GBEC) in culture. GBEC were isolated by trypsinization of small tissue fragments 
      from human gallbladders obtained at cholecystectomy; cells were plated on tissue 
      culture dishes and grown in defined MCDB 153 medium containing added growth 
      factors. In this medium, GBEC showed a plating efficiency of approximately 1%; 
      those GBEC that attached formed colonies and proliferated, as demonstrated by 
      autoradiographic analysis of [3H]thymidine incorporation into DNA. Cultured GBEC 
      expressed two markers found on GBEC in situ, i.e., gamma-glutamyl transpeptidase 
      and cytokeratin 19. By using various attachment substrates, with and without 
      added serum, increased plating efficiency and better growth were achieved. When 
      type IV collagen was used as substrate and 10% fetal bovine serum was added to 
      MCDB 153, passage of GBEC was possible, and cells proliferated through five to 
      six population doublings. GBEC in culture under all conditions eventually 
      enlarged, showed vacuolization, and demonstrated irreversible growth arrest. 
      Nonetheless, the culture conditions described here allow for preparation of large 
      quantities of highly enriched human GBEC.
FAU - Hoerl, B J
AU  - Hoerl BJ
AD  - Department of Medicine, Mayo Clinic and Foundation, Rochester, Minnesota.
FAU - Vroman, B T
AU  - Vroman BT
FAU - Kasperbauer, J L
AU  - Kasperbauer JL
FAU - LaRusso, N F
AU  - LaRusso NF
FAU - Scott, R E
AU  - Scott RE
LA  - eng
GR  - CA 28240/CA/NCI NIH HHS/United States
GR  - DK 24031/DK/NIDDK NIH HHS/United States
GR  - DK 34988/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Culture Media)
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - Cell Division
MH  - Cell Separation/methods
MH  - Cells, Cultured/*physiology
MH  - Culture Media
MH  - Culture Media, Serum-Free
MH  - Cytological Techniques
MH  - Epithelial Cells
MH  - Gallbladder/*cytology
MH  - Humans
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
PST - ppublish
SO  - Lab Invest. 1992 Feb;66(2):243-50.