PMID- 17361115 OWN - NLM STAT- MEDLINE DCOM- 20070907 LR - 20141120 IS - 0085-2538 (Print) IS - 0085-2538 (Linking) VI - 71 IP - 10 DP - 2007 May TI - Quantification of dendritic cell subsets in human renal tissue under normal and pathological conditions. PG - 1001-8 AB - Dendritic cells (DCs) play critical roles in immune responses and can be distinguished in two major subsets, myeloid and plasmacytoid DCs. Although the presence of DC in all peripheral organs, including the kidney, has been well documented, no accurate estimates of DC subsets in human kidneys have been reported. This study shows a detailed analysis of DC subsets in cryosections of human renal tissue. The cortex of normal kidneys contains at least two different HLA-DR(+) myeloid DC subtypes characterized by BDCA-1(+)DC-SIGN(+) and BDCA-1(+)DC-SIGN(-). The staining for DC-SIGN completely overlapped with CD68 in the renal interstitium. Unexpectedly, BDCA-2(+)DC-SIGN(-) plasmacytoid DCs are also abundantly present. Both subsets are located in the tubulo-interstitium often with a high frequency around, but rarely observed within glomeruli. Quantification of BDCA-1(+), DC-SIGN(+), and BDCA-2(+) cells in normal human renal tissue (pretransplant biopsy living donors; n=21) revealed that BDCA-1 is about four times as frequently present as BDCA-2. A preliminary cross-sectional analysis of DC in diseased kidneys, including rejection and immunoglobulin A nephropathy, revealed that the number of DC as well as their anatomical distribution might change under pathophysiological conditions. In conclusion, we show that human kidneys contain a dense network of myeloid and plasmacytoid DCs and provide the tools for phenotyping and enumeration of these cells to better understand interindividual differences in immune responses. FAU - Woltman, A M AU - Woltman AM AD - Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands. FAU - de Fijter, J W AU - de Fijter JW FAU - Zuidwijk, K AU - Zuidwijk K FAU - Vlug, A G AU - Vlug AG FAU - Bajema, I M AU - Bajema IM FAU - van der Kooij, S W AU - van der Kooij SW FAU - van Ham, V AU - van Ham V FAU - van Kooten, C AU - van Kooten C LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070314 PL - United States TA - Kidney Int JT - Kidney international JID - 0323470 RN - 0 (Antigens, CD1) RN - 0 (Antigens, Surface) RN - 0 (CD1C protein, human) RN - 0 (CLEC4C protein, human) RN - 0 (Cell Adhesion Molecules) RN - 0 (DC-specific ICAM-3 grabbing nonintegrin) RN - 0 (Glycoproteins) RN - 0 (Lectins, C-Type) RN - 0 (Membrane Glycoproteins) RN - 0 (Receptors, Cell Surface) RN - 0 (Receptors, Immunologic) SB - IM MH - Adult MH - Antigens, CD1 MH - Antigens, Surface/metabolism MH - Cell Adhesion Molecules/deficiency/metabolism MH - Cross-Sectional Studies MH - Dendritic Cells/*classification/cytology/metabolism/pathology MH - Fluorescent Antibody Technique MH - Glycoproteins MH - Humans MH - Immunohistochemistry/methods MH - Kidney/*cytology/*pathology MH - Kidney Diseases/*pathology MH - Kidney Transplantation MH - Lectins, C-Type/deficiency/metabolism MH - Living Donors MH - Membrane Glycoproteins/deficiency/metabolism MH - Middle Aged MH - Myeloid Cells/cytology MH - Plasma Cells/cytology MH - Receptors, Cell Surface/deficiency/metabolism MH - Receptors, Immunologic/deficiency/metabolism MH - Staining and Labeling EDAT- 2007/03/16 09:00 MHDA- 2007/09/08 09:00 CRDT- 2007/03/16 09:00 PHST- 2007/03/16 09:00 [pubmed] PHST- 2007/09/08 09:00 [medline] PHST- 2007/03/16 09:00 [entrez] AID - S0085-2538(15)52251-5 [pii] AID - 10.1038/sj.ki.5002187 [doi] PST - ppublish SO - Kidney Int. 2007 May;71(10):1001-8. doi: 10.1038/sj.ki.5002187. Epub 2007 Mar 14.