PMID- 17361326
OWN - NLM
STAT- MEDLINE
DCOM- 20070830
LR  - 20081121
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 152
IP  - 7
DP  - 2007
TI  - Direct inoculation of RNA transcripts from an infectious cDNA clone of porcine 
      reproductive and respiratory syndrome virus (PRRSV) into the lymph nodes and 
      tonsils of pigs initiates PRRSV infection in vivo.
PG  - 1383-7
AB  - The recent construction of PRRSV infectious cDNA clones affords the opportunity 
      for structural and functional studies of PRRSV genes. However, the inherent 
      instability of the PRRSV genome, the requirement of cell culture propagation, and 
      poor virus recovery have limited the usefulness of the PRRSV reverse genetics 
      system for in vivo studies. Here, we report a unique strategy of infecting pigs 
      by bypassing the traditional in vitro cell culture step required for in vivo 
      studies. We demonstrate that inoculation of RNA transcripts of a PRRSV infectious 
      cDNA clone directly into the lymph nodes and tonsils of pigs produces active 
      PRRSV infection. The information from this study will have significant 
      implications for the study of the molecular mechanism of PRRSV pathogenesis using 
      the reverse genetics system.
FAU - Key, K F
AU  - Key KF
AD  - Center for Molecular Medicine and Infectious Diseases, Department of Biomedical 
      Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA 24061-0342, USA.
FAU - DiCristina, J
AU  - DiCristina J
FAU - Gillespie, J
AU  - Gillespie J
FAU - Guenette, D K
AU  - Guenette DK
FAU - Meng, X J
AU  - Meng XJ
LA  - eng
PT  - Journal Article
DEP - 20070315
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - DNA, Viral/genetics
MH  - Lymph Nodes/virology
MH  - Palatine Tonsil/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*etiology/immunology/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/*pathogenicity
MH  - RNA, Viral/*genetics
MH  - Sus scrofa
MH  - Transcription, Genetic
EDAT- 2007/03/16 09:00
MHDA- 2007/08/31 09:00
CRDT- 2007/03/16 09:00
PHST- 2007/01/12 00:00 [received]
PHST- 2007/02/07 00:00 [accepted]
PHST- 2007/03/16 09:00 [pubmed]
PHST- 2007/08/31 09:00 [medline]
PHST- 2007/03/16 09:00 [entrez]
AID - 10.1007/s00705-007-0955-8 [doi]
PST - ppublish
SO  - Arch Virol. 2007;152(7):1383-7. doi: 10.1007/s00705-007-0955-8. Epub 2007 Mar 15.