PMID- 17362264
OWN - NLM
STAT- MEDLINE
DCOM- 20070706
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 148
IP  - 3
DP  - 2007 Jun
TI  - Potato lectin activates basophils and mast cells of atopic subjects by its 
      interaction with core chitobiose of cell-bound non-specific immunoglobulin E.
PG  - 391-401
AB  - A major factor in non-allergic food hypersensitivity could be the interaction of 
      dietary lectins with mast cells and basophils. Because immunoglobulin E (IgE) 
      contains 10-12% carbohydrates, lectins can activate and degranulate these cells 
      by cross-linking the glycans of cell-bound IgE. The present objective focuses on 
      the effect of potato lectin (Solanum tuberosum agglutinin; STA) for its ability 
      to release histamine from basophils in vitro and mast cells in vivo from 
      non-atopic and atopic subjects. In this study, subjects were selected randomly 
      based on case history and skin prick test responses with food, pollen and house 
      dust mite extracts. Skin prick test (SPT) was performed with STA at 100 microg/ml 
      concentration. Histamine release was performed using leucocytes from non-atopic 
      and atopic subjects and rat peritoneal exudate cells. SPT on 110 atopic subjects 
      using STA showed 39 subjects positive (35%); however, none showed STA-specific 
      IgE; among 20 non-atopic subjects, none were positive by SPT. Maximal histamine 
      release was found to be 65% in atopic subjects (n = 7) compared to 28% in 
      non-atopic subjects (n = 5); the release was inhibited specifically by oligomers 
      of N-acetylglucosamine and correlates well with serum total IgE levels (R(2) = 
      0.923). Binding of STA to N-linked glycoproteins (horseradish peroxidase, avidin 
      and IgG) was positive by dot blot and binding assay. As potato lectin activates 
      and degranulates both mast cells and basophils by interacting with the chitobiose 
      core of IgE glycans, higher intake of potato may increase the clinical symptoms 
      as a result of non-allergic food hypersensitivity in atopic subjects.
FAU - Pramod, S N
AU  - Pramod SN
AD  - Department of Biochemistry and Nutrition, Central Food Technological Research 
      Institute, Mysore, Karnataka, India.
FAU - Venkatesh, Y P
AU  - Venkatesh YP
FAU - Mahesh, P A
AU  - Mahesh PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070315
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Disaccharides)
RN  - 0 (Glycoproteins)
RN  - 0 (Plant Lectins)
RN  - 0 (potato lectin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 577-76-4 (chitobiose)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/immunology
MH  - Basophils/*immunology
MH  - Disaccharides/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Food Hypersensitivity/immunology
MH  - Glycoproteins/metabolism
MH  - Histamine Release
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Immunoglobulin E/blood/*metabolism
MH  - Male
MH  - Mast Cells/*immunology
MH  - Plant Lectins/*immunology
MH  - Rats
MH  - Skin Tests/methods
PMC - PMC1941928
EDAT- 2007/03/17 09:00
MHDA- 2007/07/07 09:00
PMCR- 2008/06/01
CRDT- 2007/03/17 09:00
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/07/07 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - CEI3368 [pii]
AID - 10.1111/j.1365-2249.2007.03368.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2007 Jun;148(3):391-401. doi: 10.1111/j.1365-2249.2007.03368.x. 
      Epub 2007 Mar 15.