PMID- 17362384
OWN - NLM
STAT- MEDLINE
DCOM- 20070606
LR  - 20111117
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 57
IP  - 4
DP  - 2007 Apr
TI  - Possible roles of KIR2DL4 expression on uNK cells in human pregnancy.
PG  - 233-42
AB  - PROBLEM: To investigate possible roles of the natural killer (NK) cell receptor 
      killer immunoglobulin-like receptor (KIR)2DL4 expressed on uterine NK (uNK) cells 
      during pregnancy, we investigated KIR2DL4 expression on uNK cells isolated from 
      patients with early recurrent spontaneous abortion (RSA) and normal early 
      pregnancy women, and functions of KIR2DL4 was analyzed in vitro. METHODS OF THE 
      STUDY: Semi-quantitative RT-PCR analysis was introduced to detect KIR2DL4 
      messenger RNA (mRNA) expression on uNK cells. Cytotoxicity and cytokine 
      production as the result of interaction of KIR2DL4 and its ligand human leukocyte 
      antigen (HLA)-G were analyzed in vitro with lactic dehydrogenase releasing method 
      and enzyme-linked immunosorbent assay, respectively. RESULTS: No significant 
      difference in KIR2DL4 mRNA expression was observed, while the KIR2DL4 protein 
      level in isolated uNK cells is much higher in normal controls than that in RSA 
      patients. Data showed that HLA-G transfection could not reverse the lysis of uNK 
      against HLA-G transfected K562 cells but induced cytokine production. 
      Furthermore, we demonstrated that, via KIR2DL4, membrane-bound HLA-G could induce 
      high cytotoxicity and cytokine production in a high cytotoxic, IL-2 dependent 
      human NK cell line NK-92 cells. CONCLUSION: Our data suggest that KIR2DL4 might 
      play a crucial implication for human pregnancy.
FAU - Yan, Wei-Hua
AU  - Yan WH
AD  - Medical Research Center, Taizhou Hospital of Zhejiang Province, Wenzhou Medical 
      College, Linhai, Zhejiang, People's Republic of China. yanwhcom@yahoo.com
FAU - Lin, Aifen
AU  - Lin A
FAU - Chen, Bao-Guo
AU  - Chen BG
FAU - Zhou, Mei-Ying
AU  - Zhou MY
FAU - Dai, Mei-Zhen
AU  - Dai MZ
FAU - Chen, Xue-Jiao
AU  - Chen XJ
FAU - Gan, Ling-Hong
AU  - Gan LH
FAU - Zhu, Min
AU  - Zhu M
FAU - Shi, Wei-Wu
AU  - Shi WW
FAU - Li, Bo-Li
AU  - Li BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (KIR2DL4 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
RN  - 0 (Receptors, KIR2DL4)
SB  - IM
MH  - Abortion, Habitual/*immunology/metabolism
MH  - Cell Line
MH  - Cytokines/biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - HLA Antigens/immunology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Killer Cells, Natural/immunology/*metabolism
MH  - Pregnancy/*immunology/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Immunologic/immunology/*metabolism
MH  - Receptors, KIR
MH  - Receptors, KIR2DL4
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Uterus/*immunology/metabolism
EDAT- 2007/03/17 09:00
MHDA- 2007/06/07 09:00
CRDT- 2007/03/17 09:00
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/07 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
AID - AJI469 [pii]
AID - 10.1111/j.1600-0897.2007.00469.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2007 Apr;57(4):233-42. doi: 
      10.1111/j.1600-0897.2007.00469.x.