PMID- 17362875
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20220316
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1768
IP  - 5
DP  - 2007 May
TI  - Monitoring the looping up of acyl chain labeled NBD lipids in membranes as a 
      function of membrane phase state.
PG  - 1258-67
AB  - Lipids that are labeled with the NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) group 
      are widely used as fluorescent analogues of native lipids in biological and model 
      membranes to monitor a variety of processes. The NBD group of acyl chain labeled 
      NBD lipids is known to loop up to the membrane interface in fluid phase 
      membranes. However, the organization of these lipids in gel phase membranes is 
      not resolved. In this paper, we monitored the influence of the membrane phase 
      state on the looping up behavior of acyl chain labeled NBD lipids utilizing red 
      edge excitation shift (REES) and other sensitive fluorescence approaches. 
      Interestingly, our REES results indicate that NBD group of lipids, which are 
      labeled at the fatty acyl region, resides in the more hydrophobic region in gel 
      phase membranes, and complete looping of the NBD group occurs only in the fluid 
      phase. This is supported by other fluorescence parameters such as polarization 
      and lifetime. Taken together, our results demonstrate that membrane packing, 
      which depends on temperature and the phase state of the membrane, significantly 
      affects the localization of acyl chain labeled NBD lipids. In view of the wide 
      ranging use of NBD-labeled lipids in cell and membrane biology, these results 
      could have potentially important implications in future studies involving these 
      lipids as tracers.
FAU - Raghuraman, H
AU  - Raghuraman H
AD  - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
FAU - Shrivastava, Sandeep
AU  - Shrivastava S
FAU - Chattopadhyay, Amitabha
AU  - Chattopadhyay A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070209
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (7-nitrobenz-2-oxa-1,3-diazol-4-yl)
RN  - 0 (Azoles)
RN  - 0 (Gels)
RN  - 0 (Lipids)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Nitrobenzenes)
SB  - IM
MH  - Azoles/*chemistry/*metabolism
MH  - Fluorescence
MH  - Fluorescence Polarization
MH  - Gels
MH  - Lipids/*chemistry
MH  - *Membranes, Artificial
MH  - Models, Biological
MH  - Molecular Conformation
MH  - Nitrobenzenes/*chemistry/*metabolism
MH  - *Phase Transition
MH  - Staining and Labeling
MH  - Temperature
EDAT- 2007/03/17 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/03/17 09:00
PHST- 2006/11/01 00:00 [received]
PHST- 2007/01/21 00:00 [revised]
PHST- 2007/02/01 00:00 [accepted]
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
AID - S0005-2736(07)00037-5 [pii]
AID - 10.1016/j.bbamem.2007.02.001 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2007 May;1768(5):1258-67. doi: 
      10.1016/j.bbamem.2007.02.001. Epub 2007 Feb 9.