PMID- 17364026
OWN - NLM
STAT- MEDLINE
DCOM- 20070604
LR  - 20220409
IS  - 0021-9738 (Print)
IS  - 1558-8238 (Electronic)
IS  - 0021-9738 (Linking)
VI  - 117
IP  - 4
DP  - 2007 Apr
TI  - Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and 
      recruitment to inflammatory sites.
PG  - 902-9
AB  - Monocyte recruitment to sites of inflammation is regulated by members of the 
      chemokine family of chemotactic cytokines. However, the mechanisms that govern 
      the migration of monocytes from bone marrow to blood and from blood to inflamed 
      tissues are not well understood. Here we report that CC chemokine receptor 2 
      (CCR2) is highly expressed on a subpopulation of blood monocytes whose numbers 
      are markedly decreased in CCR2(-/-) mice. In bone marrow, however, CCR2(-/-) mice 
      had an increased number of monocytes, suggesting that CCR2 is critical for 
      monocyte egress. Intravenous infusion of ex vivo-labeled WT or CCR2(-/-) bone 
      marrow into WT recipient mice demonstrated that CCR2 is necessary for efficient 
      monocyte recruitment from the blood to inflamed tissue. Analysis of mice lacking 
      monocyte chemoattractant protein-1 (MCP-1), MCP-3, MCP-5, or MCP-2 plus MCP-5 
      revealed that MCP-3 and MCP-1 are the CCR2 agonists most critical for the 
      maintenance of normal blood monocyte counts. These findings provide evidence that 
      CCR2 and MCP-3/MCP-1 are critical for monocyte mobilization and suggest new roles 
      for monocyte chemoattractants in leukocyte homeostasis.
FAU - Tsou, Chia-Lin
AU  - Tsou CL
AD  - Gladstone Institute of Cardiovascular Disease, UCSF, San Francisco, California 
      94158, USA.
FAU - Peters, Wendy
AU  - Peters W
FAU - Si, Yue
AU  - Si Y
FAU - Slaymaker, Sarah
AU  - Slaymaker S
FAU - Aslanian, Ara M
AU  - Aslanian AM
FAU - Weisberg, Stuart P
AU  - Weisberg SP
FAU - Mack, Matthias
AU  - Mack M
FAU - Charo, Israel F
AU  - Charo IF
LA  - eng
GR  - R01 HL052773/HL/NHLBI NIH HHS/United States
GR  - R01 HL063894/HL/NHLBI NIH HHS/United States
GR  - 5R01 HL52773-12/HL/NHLBI NIH HHS/United States
GR  - R01 HL63894-07/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070315
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (CCL7 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Ccl7 protein, mouse)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL7)
RN  - 0 (Chemokines)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Blood Cell Count
MH  - Bone Marrow Cells/*physiology
MH  - Bone Marrow Transplantation/physiology
MH  - Chemokine CCL7
MH  - Chemokines/blood
MH  - Humans
MH  - Inflammation/*physiopathology
MH  - Mice
MH  - Mice, Knockout
MH  - Monocyte Chemoattractant Proteins/genetics/*physiology
MH  - Monocytes/immunology/*physiology
MH  - Polymorphism, Genetic
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/deficiency/genetics/*physiology
PMC - PMC1810572
EDAT- 2007/03/17 09:00
MHDA- 2007/06/05 09:00
PMCR- 2007/04/02
CRDT- 2007/03/17 09:00
PHST- 2006/07/31 00:00 [received]
PHST- 2007/01/24 00:00 [accepted]
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/05 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
PHST- 2007/04/02 00:00 [pmc-release]
AID - 29919 [pii]
AID - 10.1172/JCI29919 [doi]
PST - ppublish
SO  - J Clin Invest. 2007 Apr;117(4):902-9. doi: 10.1172/JCI29919. Epub 2007 Mar 15.