PMID- 17364073
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20221207
IS  - 0304-4602 (Print)
IS  - 0304-4602 (Linking)
VI  - 36
IP  - 2
DP  - 2007 Feb
TI  - Interleukin-18 promoter gene polymorphisms in Chinese patients with systemic 
      lupus erythematosus: association with CC genotype at position -607.
PG  - 91-5
AB  - INTRODUCTION: Interleukin-18 (IL-18) is a Th1 cytokine, which is postulated to 
      play a role in systemic lupus erythematosus (SLE). Two single nucleotide 
      polymorphisms (SNPs) in the IL-18 promoter gene region were found to influence 
      the quantitative expression of the IL-18 protein. The aim of this study was to 
      determine whether IL-18 promoter gene polymorphisms are associated with SLE. 
      MATERIALS AND METHODS: One hundred and thirteen Chinese SLE patients and 218 
      Chinese healthy individuals were recruited. Genomic DNA was extracted from 
      peripheral venous blood. Sequence-specific primer PCR and restriction fragment 
      length polymorphism (RFLP) analysis were used to genotype the DNA samples for 
      SNP-137 and SNP- 607. The following genotypes were obtained: SNP(-607) AA, AC, CC 
      and SNP(-137) GG, GC, CC. Plasma IL-18 concentrations of patients and control 
      subjects were measured by enzyme-linked immunosorbent assay. RESULTS: the 
      frequency of SNP-607/CC genotype was significantly higher in SLE patients (Pc < 
      0.05) while genotype SNP-607/AC was significantly decreased in SLE patients 
      compared to the control group (Pc <0.05). Plasma IL-18 concentrations were 
      significantly higher in SLE patients than in control subjects (P <0.05). Both 
      patients and control subjects with CC and AC genotypes have significantly higher 
      IL-18 concentrations than those with AA genotype. CONCLUSION: The IL-18 promoter 
      gene polymorphism SNP-607 C allele is associated with SLE and may result in the 
      enhanced production of IL-18 protein in SLE and normal individuals.
FAU - Xu, Qian
AU  - Xu Q
AD  - Department of Medicine, National University of Singapore, Singapore. 
      xuqian@nus.edu.sg
FAU - Tin, Soe Kyaw
AU  - Tin SK
FAU - Sivalingam, Suppiah Paramalingam
AU  - Sivalingam SP
FAU - Thumboo, Julian
AU  - Thumboo J
FAU - Koh, Dow Rhoon
AU  - Koh DR
FAU - Fong, Kok Yong
AU  - Fong KY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Singapore
TA  - Ann Acad Med Singap
JT  - Annals of the Academy of Medicine, Singapore
JID - 7503289
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Asian People/*genetics
MH  - Female
MH  - Gene Frequency
MH  - Genotype
MH  - Humans
MH  - Interleukin-18/*genetics
MH  - Lupus Erythematosus, Systemic/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
EDAT- 2007/03/17 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/03/17 09:00
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
PST - ppublish
SO  - Ann Acad Med Singap. 2007 Feb;36(2):91-5.