PMID- 17364223
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20220317
IS  - 0167-4412 (Print)
IS  - 0167-4412 (Linking)
VI  - 64
IP  - 1-2
DP  - 2007 May
TI  - Overexpression of salicylic acid carboxyl methyltransferase reduces salicylic 
      acid-mediated pathogen resistance in Arabidopsis thaliana.
PG  - 1-15
AB  - We cloned a salicylic acid/benzoic acid carboxyl methyltransferase gene, OsBSMT1, 
      from Oryza sativa. A recombinant OsBSMT1 protein obtained by expressing the gene 
      in Escherichia coli exhibited carboxyl methyltransferase activity in reactions 
      with salicylic acid (SA), benzoic acid (BA), and de-S-methyl 
      benzo(1,2,3)thiadiazole-7-carbothioic acid (dSM-BTH), producing methyl salicylate 
      (MeSA), methyl benzoate (MeBA), and methyl dSM-BTH (MeBTH), respectively. 
      Compared to wild-type plants, transgenic Arabidopsis overexpressing OsBSMT1 
      accumulated considerably higher levels of MeSA and MeBA, some of which were 
      vaporized into the environment. Upon infection with the bacterial pathogen 
      Pseudomonas syringae or the fungal pathogen Golovinomyces orontii, transgenic 
      plants failed to accumulate SA and its glucoside (SAG), becoming more susceptible 
      to disease than wild-type plants. OsBSMT1-overexpressing Arabidopsis showed 
      little induction of PR-1 when treated with SA or G. orontii. Notably, incubation 
      with the transgenic plant was sufficient to trigger PR-1 induction in neighboring 
      wild-type plants. Together, our results indicate that in the absence of SA, MeSA 
      alone cannot induce a defense response, yet it serves as an airborne signal for 
      plant-to-plant communication. We also found that jasmonic acid (JA) induced 
      AtBSMT1, which may contribute to an antagonistic effect on SA signaling pathways 
      by depleting the SA pool in plants.
FAU - Koo, Yeon Jong
AU  - Koo YJ
AD  - School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, 
      Korea.
FAU - Kim, Myeong Ae
AU  - Kim MA
FAU - Kim, Eun Hye
AU  - Kim EH
FAU - Song, Jong Tae
AU  - Song JT
FAU - Jung, Choonkyun
AU  - Jung C
FAU - Moon, Joon-Kwan
AU  - Moon JK
FAU - Kim, Jeong-Han
AU  - Kim JH
FAU - Seo, Hak Soo
AU  - Seo HS
FAU - Song, Sang Ik
AU  - Song SI
FAU - Kim, Ju-Kon
AU  - Kim JK
FAU - Lee, Jong Seob
AU  - Lee JS
FAU - Cheong, Jong-Joo
AU  - Cheong JJ
FAU - Choi, Yang Do
AU  - Choi YD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070316
PL  - Netherlands
TA  - Plant Mol Biol
JT  - Plant molecular biology
JID - 9106343
RN  - 0 (Cyclopentanes)
RN  - 0 (Oxylipins)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Salicylates)
RN  - 6RI5N05OWW (jasmonic acid)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.- (salicylic acid carboxyl methyltransferase)
RN  - LAV5U5022Y (methyl salicylate)
RN  - O414PZ4LPZ (Salicylic Acid)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/*genetics/microbiology/physiology
MH  - Ascomycota/physiology
MH  - Cloning, Molecular
MH  - Cyclopentanes/metabolism
MH  - Immunity, Innate/genetics
MH  - Methyltransferases/*genetics/metabolism/physiology
MH  - Molecular Sequence Data
MH  - Oryza/genetics
MH  - Oxylipins
MH  - Plant Proteins/*genetics/metabolism/physiology
MH  - Plants, Genetically Modified/*enzymology/metabolism/microbiology
MH  - Pseudomonas syringae/physiology
MH  - Recombinant Proteins/metabolism
MH  - Salicylates/metabolism
MH  - Salicylic Acid/metabolism
MH  - Sequence Alignment
EDAT- 2007/03/17 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/03/17 09:00
PHST- 2006/08/03 00:00 [received]
PHST- 2006/12/07 00:00 [accepted]
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
AID - 10.1007/s11103-006-9123-x [doi]
PST - ppublish
SO  - Plant Mol Biol. 2007 May;64(1-2):1-15. doi: 10.1007/s11103-006-9123-x. Epub 2007 
      Mar 16.