PMID- 17366484
OWN - NLM
STAT- MEDLINE
DCOM- 20070606
LR  - 20070514
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 28
IP  - 8
DP  - 2007 Apr
TI  - A sheathless poly(methyl methacrylate) chip-CE/MS interface fabricated using a 
      wire-assisted epoxy-fixing method.
PG  - 1265-73
AB  - Using a wire-assisted epoxy-fixing method, a sheathless CE/MS interface on a 
      poly-(methyl methacrylate) (PMMA) CE chip has been developed. The sheathless 
      chip-CE/MS interface utilized a tapered fused-silica tip and the electrical 
      connection was achieved through a layered coating of conductive rubber. The 
      wire-assisted method provided facile alignment of channels between the PMMA CE 
      chip and an external capillary sprayer without the need for micromachining. 
      Because the wire was in the channel during fixing, the risk of channel blockage 
      by the epoxy was avoided. This chip CE device has minimal dead volume because the 
      interstitial spaces were filled by a fast-fixing epoxy resin. The performance of 
      the chip-CE-ESI-MS device was demonstrated with the analysis of peptide mixtures.
FAU - Li, Fu-An
AU  - Li FA
AD  - Department of Chemistry, National Taiwan University, Taipei, Taiwan.
FAU - Wang, Chih-Hung
AU  - Wang CH
FAU - Her, Guor-Rong
AU  - Her GR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Epoxy Resins)
RN  - 0 (Peptides)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Electroosmosis
MH  - Electrophoresis, Microchip/*instrumentation
MH  - Epoxy Resins
MH  - Equipment Design
MH  - Peptides/isolation & purification
MH  - Polymethyl Methacrylate
MH  - Spectrometry, Mass, Electrospray Ionization/instrumentation/statistics & 
      numerical data
EDAT- 2007/03/17 09:00
MHDA- 2007/06/07 09:00
CRDT- 2007/03/17 09:00
PHST- 2007/03/17 09:00 [pubmed]
PHST- 2007/06/07 09:00 [medline]
PHST- 2007/03/17 09:00 [entrez]
AID - 10.1002/elps.200600552 [doi]
PST - ppublish
SO  - Electrophoresis. 2007 Apr;28(8):1265-73. doi: 10.1002/elps.200600552.