PMID- 17367985
OWN - NLM
STAT- MEDLINE
DCOM- 20070925
LR  - 20231213
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 21
IP  - 5
DP  - 2007 Aug
TI  - Morphological study on apoptosis Hela cells induced by soyasaponins.
PG  - 820-6
AB  - Soyasaponins are present in legumes and soybeans are the primary dietary source 
      of saponins. SS-II, the second fraction of soyasaponins, was separated by column 
      chromatographic method with D101A macroporous resin from soybean. In this paper, 
      at the concentration range of 100-400 mg/L, SS-II had obvious cytotoxic effect on 
      Hela cells by MTT assay. After Hela cells were treated with SS-II, typical 
      apoptotic morphological changes, including nuclear fragmentation, cytoplasm 
      shrinkage and decrease of cell volume, were observed by fluorescence microscope, 
      transmission electron microscope (TEM) and confocal laser scanning microscope 
      (CLSM), respectively. Terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL) assay also confirmed that SS-II-treated Hela cells showed 
      apoptotic features. The results suggested that soyasaponins were a potential 
      antitumor compound and the apoptosis induced by soyasaponins was a key antitumor 
      mechanism.
FAU - Xiao, Jun-Xia
AU  - Xiao JX
AD  - School of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, PR China.
FAU - Huang, Guo-Qing
AU  - Huang GQ
FAU - Zhu, Cai-Ping
AU  - Zhu CP
FAU - Ren, Dan-Dan
AU  - Ren DD
FAU - Zhang, Sheng-Hua
AU  - Zhang SH
LA  - eng
PT  - Journal Article
DEP - 20070209
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Saponins)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Apoptosis/*drug effects
MH  - DNA Fragmentation/drug effects
MH  - DNA, Neoplasm/drug effects
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - Saponins/*pharmacology
MH  - Glycine max/*chemistry
MH  - Tetrazolium Salts
MH  - Thiazoles
EDAT- 2007/03/21 09:00
MHDA- 2007/09/26 09:00
CRDT- 2007/03/21 09:00
PHST- 2006/08/17 00:00 [received]
PHST- 2006/11/30 00:00 [revised]
PHST- 2007/01/27 00:00 [accepted]
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/09/26 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - S0887-2333(07)00050-1 [pii]
AID - 10.1016/j.tiv.2007.01.025 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2007 Aug;21(5):820-6. doi: 10.1016/j.tiv.2007.01.025. Epub 2007 
      Feb 9.