PMID- 17368810
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20161124
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 109
IP  - 2
DP  - 2007 Apr 15
TI  - Nicotine alters the biological activities of developing mouse bone marrow-derived 
      dendritic cells (DCs).
PG  - 155-64
AB  - Cigarette smoke contains nicotine, an immunomodulatory component that is thought 
      to affect immune surveillance and increase the progression of diseases. Dendritic 
      cells (DCs) constitute a family of antigen-presenting (APCs) with inherent 
      abilities to sense and translate environmental cues and to shape host immunity. 
      We recently reported on the effects of nicotine on human DCs and proposed a 
      possible mechanism that links cigarette smoke to higher incidences of respiratory 
      tract infection and asthma. To establish the causal relationship between 
      nicotine-induced DC alterations and immunomodulation in vivo, we translated our 
      in vitro human results to the mouse system and studied the direct effects of 
      nicotine exposure on the biological and functional properties of mouse bone 
      marrow (BM) DCs differentiated in vitro from their precursors. We report that 
      while the presence of nicotine in the microenvironment has no direct effect on 
      competent mouse BM-derived DCs function, it promotes the development of mouse BM 
      DC precursors into DCs (thereafter called nicDCs) with a semi-mature phenotype 
      revealed by higher expression of costimulatory molecules CD80, CD86, CD40, MHC II 
      molecules and the lymph node homing receptor, CCR7. Consistent with their 
      maturational status, these nicDCs have reduced capacity for antigen uptake and 
      produce substantially less Th1-promoting cytokine, IL-12, in response to 
      Th1-polarizing adjuvant, lipopolysaccharide (LPS). Interestingly, we found that 
      nicDCs preferentially support the proliferation and differentiation of ovalbumin 
      (OVA)-specific naive T cells into effector memory cells, producing significantly 
      less IFN-gamma and more IL-4. These results provide evidence for the similarity 
      in the effects of nicotine on mouse and human DCs, particularly the ability to 
      modulate DC differentiation towards developing Th2 immunity.
FAU - Nouri-Shirazi, M
AU  - Nouri-Shirazi M
AD  - Department of Biomedical Sciences, Immunology Laboratory, Texas A&M University 
      System Health Science Center, Baylor College of Dentistry, Dallas, TX 75246, USA. 
      mnshirazi@bcd.tamhsc.edu
FAU - Tinajero, R
AU  - Tinajero R
FAU - Guinet, E
AU  - Guinet E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070309
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Dextrans)
RN  - 0 (fluorescein isothiocyanate dextran)
RN  - 6M3C89ZY6R (Nicotine)
RN  - 9006-59-1 (Ovalbumin)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects/immunology
MH  - Cell Survival/drug effects
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Dextrans/metabolism
MH  - Fluorescein-5-isothiocyanate/analogs & derivatives/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Transgenic
MH  - Nicotine/*pharmacology
MH  - Ovalbumin/metabolism
MH  - T-Lymphocytes/drug effects/immunology
EDAT- 2007/03/21 09:00
MHDA- 2007/08/24 09:00
CRDT- 2007/03/21 09:00
PHST- 2006/12/22 00:00 [received]
PHST- 2007/02/03 00:00 [revised]
PHST- 2007/02/10 00:00 [accepted]
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - S0165-2478(07)00034-X [pii]
AID - 10.1016/j.imlet.2007.02.005 [doi]
PST - ppublish
SO  - Immunol Lett. 2007 Apr 15;109(2):155-64. doi: 10.1016/j.imlet.2007.02.005. Epub 
      2007 Mar 9.