PMID- 17369104
OWN - NLM
STAT- MEDLINE
DCOM- 20070827
LR  - 20131121
IS  - 1566-0702 (Print)
IS  - 1566-0702 (Linking)
VI  - 134
IP  - 1-2
DP  - 2007 Jul 31
TI  - D-beta-hydroxybutyrate inhibits the apoptosis of PC12 cells induced by 6-OHDA in 
      relation to up-regulating the ratio of Bcl-2/Bax mRNA.
PG  - 38-44
AB  - D-beta-hydroxybutyrate (DbetaHB) is a predominant member of ketone bodies 
      produced by hepatocytes and, to a lesser extent, by astrocytes. It is an 
      alternative source of energy in the brain when glucose supply is depleted such as 
      during starvation. It has been reported that ketone bodies could protect 
      dopaminergic culture. However, the biological function of DbetaHB in Parkinson 
      disease (PD) is still unclear. In the present work, we investigated the role of 
      DbetaHB in protecting rat pheochromocytoma (PC12) cells from apoptosis induced by 
      6-Hydroxydopamine (6-OHDA). DbetaHB rescued PC12 cells from apoptotic death 
      induced by 6-OHDA by MTT assay, acridine orange (AO) staining, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and 
      the activity of caspase-3. DbetaHB prevented the decrease of cell viability and 
      the increase of caspase-3 activity induced by 6-OHDA in a dose-dependent manner 
      in PC12 cells. AO and TUNEL staining showed that DbetaHB prevented the apoptosis 
      of PC12 cells induced by 6-OHDA. The ratio of Bcl-2/Bax at mRNA levels, which 
      regulates the apoptosis of PC12 cells when exposed to 6-OHDA, increased when 
      DbetaHB was preincubated. The data showed that DbetaHB inhibited the apoptosis of 
      PC12 cells induced by 6-OHDA in relation to up-regulating the ratio of Bcl-2/Bax 
      mRNA.
FAU - Cheng, Baohua
AU  - Cheng B
AD  - Shandong University, Jinan, China. chengbh1979@163.com
FAU - Yang, Xinxin
AU  - Yang X
FAU - Hou, Zhongyu
AU  - Hou Z
FAU - Lin, Xiangtao
AU  - Lin X
FAU - Meng, Haiwei
AU  - Meng H
FAU - Li, Zhenping
AU  - Li Z
FAU - Liu, Shuwei
AU  - Liu S
LA  - eng
PT  - Journal Article
DEP - 20070321
PL  - Netherlands
TA  - Auton Neurosci
JT  - Autonomic neuroscience : basic & clinical
JID - 100909359
RN  - 0 (Bax protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sympatholytics)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 3.4.22.- (Caspase 3)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/*pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects/physiology
MH  - Caspase 3/metabolism
MH  - Cell Survival/drug effects/physiology
MH  - Drug Interactions
MH  - Gene Expression Regulation/drug effects
MH  - Microscopy/methods
MH  - Neurons/cytology/*drug effects/physiology
MH  - Oxidopamine/*pharmacology
MH  - PC12 Cells
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Sympatholytics/*pharmacology
MH  - Up-Regulation/drug effects/physiology
MH  - bcl-2-Associated X Protein/*genetics
EDAT- 2007/03/21 09:00
MHDA- 2007/08/28 09:00
CRDT- 2007/03/21 09:00
PHST- 2006/11/20 00:00 [received]
PHST- 2007/02/06 00:00 [revised]
PHST- 2007/02/06 00:00 [accepted]
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/08/28 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - S1566-0702(07)00043-4 [pii]
AID - 10.1016/j.autneu.2007.02.002 [doi]
PST - ppublish
SO  - Auton Neurosci. 2007 Jul 31;134(1-2):38-44. doi: 10.1016/j.autneu.2007.02.002. 
      Epub 2007 Mar 21.