PMID- 17369294
OWN - NLM
STAT- MEDLINE
DCOM- 20070803
LR  - 20131121
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 22
IP  - 6
DP  - 2007 Jun
TI  - Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed 
      by acridine orange fluorescence.
PG  - 1597-602
AB  - BACKGROUND: The aim of this study was to determine if only sperm with double 
      stranded DNA could bind to the human zona pellucida (ZP). METHODS: Sperm samples 
      from 124 infertile men with a range of semen abnormalities were studied. Oocytes 
      that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding 
      test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile 
      sperm selected by colloidal silica gradient centrifugation (CSGC). After 
      assessing the number of sperm bound per ZP, all sperm bound to the surface of the 
      ZP of four oocytes were dislodged and placed on a glass slide. The double (green 
      fluorescence) or single stranded (denatured, red fluorescence) DNA of sperm in 
      semen, motile sperm selected by CSGC and ZP-bound sperm, was assessed by acridine 
      orange (AO) fluorescence. RESULTS: The percentage of sperm with green 
      fluorescence was significantly correlated with normal sperm morphology in semen 
      and after CSGC preparation. The proportion of sperm with green fluorescence was 
      significantly higher in motile sperm selected by CSGC than ejaculated sperm. 
      There were very few sperm (average <8%) with red fluorescence bound to the ZP, 
      even in men who had very high (>70%) proportions of sperm with red fluorescence 
      in their semen. CONCLUSION: Sperm binding to human ZP is highly selective for 
      double stranded DNA. Sperm with single stranded or denatured DNA bind less or do 
      not bind at all to the ZP, probably because of defects of motility and, more 
      especially, morphology.
FAU - Liu, D Y
AU  - Liu DY
AD  - Department of Obstetrics and Gynaecology, University of Melbourne, Reproductive 
      Services and Melbourne IVF, Royal Women's Hospital, Victoria 3053, Australia. 
      dy1@unimelb.edu.au
FAU - Baker, H W G
AU  - Baker HW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070316
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/analysis
MH  - Adult
MH  - Chromatin/chemistry/metabolism
MH  - DNA/analysis/*metabolism
MH  - Female
MH  - Fluorescence
MH  - Humans
MH  - Infertility, Male
MH  - Male
MH  - Middle Aged
MH  - Sperm Motility
MH  - *Sperm-Ovum Interactions
MH  - Spermatozoa/chemistry/cytology/*metabolism
MH  - Zona Pellucida/*metabolism
EDAT- 2007/03/21 09:00
MHDA- 2007/08/04 09:00
CRDT- 2007/03/21 09:00
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/08/04 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - dem044 [pii]
AID - 10.1093/humrep/dem044 [doi]
PST - ppublish
SO  - Hum Reprod. 2007 Jun;22(6):1597-602. doi: 10.1093/humrep/dem044. Epub 2007 Mar 
      16.