PMID- 17371954
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 178
IP  - 7
DP  - 2007 Apr 1
TI  - Cutting Edge: Immature human dendritic cells express latency-associated peptide 
      and inhibit T cell activation in a TGF-beta-dependent manner.
PG  - 4017-21
AB  - Dendritic cells (DCs) play a critical role in both initiating immune responses 
      and in maintaining peripheral tolerance. However, the exact mechanism by which 
      DCs instruct/influence the generation of effector vs regulatory T cells is not 
      clear. In this study, we present evidence that TGF-beta, an important 
      immunoregulatory molecule, is present on the surface of ex vivo immature human 
      DCs bound by latency-associated peptide (LAP). Maturation of DCs upon stimulation 
      with LPS results in loss of membrane-bound LAP and up-regulation of HLA class II 
      and costimulatory molecules. The presence of LAP on immature DCs selectively 
      inhibits Th1 cell but not Th17 cell differentiation and is required for 
      differentiation and/or survival of Foxp3-positive regulatory T cells. Taken 
      together, our results indicate that surface expression of TGF-beta on DCs in 
      association with LAP is one of the mechanisms by which immature DCs limit T cell 
      activation and thus prevent autoimmune responses.
FAU - Gandhi, Roopali
AU  - Gandhi R
AD  - Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical 
      School, Boston, MA 02115, USA.
FAU - Anderson, David E
AU  - Anderson DE
FAU - Weiner, Howard L
AU  - Weiner HL
LA  - eng
GR  - NS 23132/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies)
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Transforming Growth Factor beta)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Autoimmunity
MH  - Cell Differentiation
MH  - Cell Polarity
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Dendritic Cells/chemistry/drug effects/*immunology
MH  - Forkhead Transcription Factors/analysis/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - RNA, Small Interfering/genetics
MH  - T-Lymphocytes/*immunology
MH  - T-Lymphocytes, Regulatory/immunology
MH  - Th1 Cells/immunology
MH  - Transforming Growth Factor beta/analysis/antagonists & inhibitors/*metabolism
EDAT- 2007/03/21 09:00
MHDA- 2007/06/20 09:00
CRDT- 2007/03/21 09:00
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - 178/7/4017 [pii]
AID - 10.4049/jimmunol.178.7.4017 [doi]
PST - ppublish
SO  - J Immunol. 2007 Apr 1;178(7):4017-21. doi: 10.4049/jimmunol.178.7.4017.