PMID- 17372317
OWN - NLM
STAT- MEDLINE
DCOM- 20070515
LR  - 20080121
IS  - 1930-7381 (Print)
IS  - 1930-7381 (Linking)
VI  - 15
IP  - 3
DP  - 2007 Mar
TI  - Monocyte chemoattractant protein-1 in obesity and type 2 diabetes. Insulin 
      sensitivity study.
PG  - 664-72
AB  - OBJECTIVE: Our goal was to test any association between human plasma circulating 
      levels of monocyte chemoattractant protein-1 (cMCP-1) and insulin resistance and 
      to compare monocyte chemoattractant protein-1 (MCP-1) adipose tissue gene 
      expression and cMCP-1 in relation with inflammatory markers. RESEARCH METHODS AND 
      PROCEDURES: cMCP-1 was measured in n = 116 consecutive control male subjects to 
      whom an insulin sensitivity (S(i)) test was performed. Circulating levels of 
      soluble CD14, soluble tumor necrosis factor receptor type 2 (sTNFR2), soluble 
      interleukin-6 (sIL-6), and adiponectin also were measured. Subcutaneous adipose 
      tissue samples were obtained from n = 107 non-diabetic and type 2 diabetic 
      subjects with different degrees of obesity. Real-time polymerase chain reaction 
      was used to measure gene expression of MCP-1, CD68, tumor necrosis factor-alpha 
      (TNF-alpha), and its receptor TNFR2. RESULTS: In the S(i) study, no independent 
      effect of cMCP-1 levels on insulin sensitivity was observed. In the expression 
      study, in non-diabetic subjects, MCP-1 mRNA had a positive correlation with BMI 
      (r = 0.407, p = 0.003), TNF-alpha mRNA (r = 0.419, p = 0.002), and TNFR2 mRNA (r 
      = 0.410, p = 0.003). In these subjects, cMCP-1 was found to correlate with 
      waist-to-hip ratio (r = 0.322, p = 0.048). In patients with type 2 diabetes, 
      MCP-1 mRNA was up-regulated compared with non-diabetic subjects. TNF-alpha mRNA 
      was found to independently contribute to MCP-1 mRNA expression. In this group, 
      CD68 mRNA was found to correlate with BMI (r = 0.455, p = 0.001). DISCUSSION: 
      cMCP-1 is not associated with insulin sensitivity in apparently healthy men. 
      TNF-alpha is the inflammatory cytokine associated with MCP-1 expression in 
      subcutaneous adipose tissue.
FAU - Chacon, Matilde R
AU  - Chacon MR
AD  - Research Department, University Hospital of Tarragona Joan XXIII, Rovira i 
      Virgili University, Pere Virgili Institute, Tarragona, Spain.
FAU - Fernandez-Real, Jose Manuel
AU  - Fernandez-Real JM
FAU - Richart, Cristobal
AU  - Richart C
FAU - Megia, Ana
AU  - Megia A
FAU - Gomez, Jose Manuel
AU  - Gomez JM
FAU - Miranda, Merce
AU  - Miranda M
FAU - Caubet, Enric
AU  - Caubet E
FAU - Pastor, Rosa
AU  - Pastor R
FAU - Masdevall, Carles
AU  - Masdevall C
FAU - Vilarrasa, Nuria
AU  - Vilarrasa N
FAU - Ricard, Wifredo
AU  - Ricard W
FAU - Vendrell, Joan
AU  - Vendrell J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Obesity (Silver Spring)
JT  - Obesity (Silver Spring, Md.)
JID - 101264860
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Body Mass Index
MH  - Case-Control Studies
MH  - Chemokine CCL2/*blood/genetics/metabolism
MH  - Diabetes Mellitus, Type 2/*blood/genetics/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Insulin Resistance/*physiology
MH  - Male
MH  - Middle Aged
MH  - Obesity/*blood/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Subcutaneous Fat/metabolism
EDAT- 2007/03/21 09:00
MHDA- 2007/05/16 09:00
CRDT- 2007/03/21 09:00
PHST- 2007/03/21 09:00 [pubmed]
PHST- 2007/05/16 09:00 [medline]
PHST- 2007/03/21 09:00 [entrez]
AID - 15/3/664 [pii]
AID - 10.1038/oby.2007.578 [doi]
PST - ppublish
SO  - Obesity (Silver Spring). 2007 Mar;15(3):664-72. doi: 10.1038/oby.2007.578.