PMID- 17373456
OWN - NLM
STAT- MEDLINE
DCOM- 20070606
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 90
IP  - 1
DP  - 2007 Jan-Feb
TI  - Optimized determination method for trans-10-hydroxy-2-decenoic acid content in 
      royal jelly by high-performance liquid chromatography with an internal standard.
PG  - 244-9
AB  - An optimized reversed-phase high-performance liquid chromatography method was 
      developed to detect the trans-10-hydroxy-2-decenoic acid (10-HDA) content in 
      royal jelly cream and lyophilized powder. The sample was extracted using absolute 
      ethanol. Chromatographic separation of 10-HDA and methyl 4-hydroxybenzoate as the 
      internal standard was performed on a Nova-pak C18 column. The average recoveries 
      were 95.0-99.2% (n = 5) with relative standard deviation (RSD) values of 1.3-2.1% 
      for royal jelly cream and 98.0-100.0% (n = 5) with RSD values of 1.6-3.0% for 
      lyophilized powder, respectively. The limits of detection and quantitation were 
      0.5 and 1.5 mg/kg, respectively, for both royal jelly cream and lyophilized 
      powder. The method was validated for the determination of practical royal jelly 
      products. The concentration of 10-HDA ranged from 1.26 to 2.21% for pure royal 
      jelly cream samples and 3.01 to 6.19% for royal jelly lyophilized powder samples. 
      For 30 royal jelly products, the 10-HDA content varied from not detectable to 
      0.98%.
FAU - Zhou, Jinhui
AU  - Zhou J
AD  - Beeproduct Quality Supervision and Inspection Testing Center, Ministry of 
      Agriculture, Beijing 100093, People's Republic of China.
FAU - Xue, Xiaofeng
AU  - Xue X
FAU - Li, Yi
AU  - Li Y
FAU - Zhang, Jinzhen
AU  - Zhang J
FAU - Zhao, Jing
AU  - Zhao J
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Fatty Acids)
RN  - 0 (Fatty Acids, Monounsaturated)
RN  - 0 (Indicators and Reagents)
RN  - 765-01-5 (10-hydroxy-2-decenoic acid)
RN  - L497I37F0C (royal jelly)
SB  - IM
MH  - Animals
MH  - Bees
MH  - Chromatography, High Pressure Liquid/methods
MH  - Fatty Acids/*chemistry
MH  - Fatty Acids, Monounsaturated/*analysis
MH  - Freeze Drying
MH  - Indicators and Reagents
MH  - Kinetics
EDAT- 2007/03/22 09:00
MHDA- 2007/06/07 09:00
CRDT- 2007/03/22 09:00
PHST- 2007/03/22 09:00 [pubmed]
PHST- 2007/06/07 09:00 [medline]
PHST- 2007/03/22 09:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 2007 Jan-Feb;90(1):244-9.