PMID- 17376002
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20220321
IS  - 0897-7151 (Print)
IS  - 0897-7151 (Linking)
VI  - 24
IP  - 2
DP  - 2007 Feb
TI  - Axonal remyelination by cord blood stem cells after spinal cord injury.
PG  - 391-410
AB  - Human umbilical cord blood stem cells (hUCB) hold great promise for therapeutic 
      repair after spinal cord injury (SCI). Here, we present our preliminary 
      investigations on axonal remyelination of injured spinal cord by transplanted 
      hUCB. Adult male rats were subjected to moderate SCI using NYU Impactor, and hUCB 
      were grafted into the site of injury one week after SCI. Immunohistochemical data 
      provides evidence of differentiation of hUCB into several neural phenotypes 
      including neurons, oligodendrocytes and astrocytes. Ultrastructural analysis of 
      axons reveals that hUCB form morphologically normal appearing myelin sheaths 
      around axons in the injured areas of spinal cord. Colocalization studies prove 
      that oligodendrocytes derived from hUCB secrete neurotrophic hormones 
      neurotrophin-3 (NT3) and brain-derived neurotrophic factor (BDNF). Cord blood 
      stem cells aid in the synthesis of myelin basic protein (MBP) and proteolipid 
      protein (PLP) of myelin in the injured areas, thereby facilitating the process of 
      remyelination. Elevated levels of mRNA expression were observed for NT3, BDNF, 
      MBP and PLP in hUCB-treated rats as revealed by fluorescent in situ hybridization 
      (FISH) analysis. Recovery of hind limb locomotor function was also significantly 
      enhanced in the hUCB-treated rats based on Basso-Beattie-Bresnahan (BBB) scores 
      assessed 14 days after transplantation. These findings demonstrate that hUCB, 
      when transplanted into the spinal cord 7 days after weight-drop injury, survive 
      for at least 2 weeks, differentiate into oligodendrocytes and neurons, and enable 
      improved locomotor function. Therefore, hUCB facilitate functional recovery after 
      moderate SCI and may prove to be a useful therapeutic strategy to repair the 
      injured spinal cord.
FAU - Dasari, Venkata Ramesh
AU  - Dasari VR
AD  - Program of Cancer Biology, Department of Cancer Biology and Pharmacology, 
      University of Illinois College of Medicine at Peoria, Peoria, Illinois 61605, 
      USA.
FAU - Spomar, Daniel G
AU  - Spomar DG
FAU - Gondi, Christopher S
AU  - Gondi CS
FAU - Sloffer, Christopher A
AU  - Sloffer CA
FAU - Saving, Kay L
AU  - Saving KL
FAU - Gujrati, Meena
AU  - Gujrati M
FAU - Rao, Jasti S
AU  - Rao JS
FAU - Dinh, Dzung H
AU  - Dinh DH
LA  - eng
GR  - R01 NS057529/NS/NINDS NIH HHS/United States
GR  - NS 47699/NS/NINDS NIH HHS/United States
GR  - R01 CA075557/CA/NCI NIH HHS/United States
GR  - NS 57529/NS/NINDS NIH HHS/United States
GR  - CA 75557/CA/NCI NIH HHS/United States
GR  - CA 92393/CA/NCI NIH HHS/United States
GR  - CA 95058/CA/NCI NIH HHS/United States
GR  - R01 CA116708/CA/NCI NIH HHS/United States
GR  - R01 NS047699/NS/NINDS NIH HHS/United States
GR  - R01 CA095058/CA/NCI NIH HHS/United States
GR  - R01 CA092393/CA/NCI NIH HHS/United States
GR  - CA 116708/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - *Cord Blood Stem Cell Transplantation
MH  - Hindlimb/physiopathology
MH  - Humans
MH  - Male
MH  - Motor Activity/physiology
MH  - Myelin Sheath/*physiology
MH  - Nerve Growth Factors/metabolism
MH  - Rats
MH  - Recovery of Function/physiology
MH  - Regeneration/*physiology
MH  - Spinal Cord Injuries/metabolism/*physiopathology/*therapy
MH  - Thoracic Vertebrae
PMC - PMC1859845
MID - NIHMS14242
EDAT- 2007/03/23 09:00
MHDA- 2007/06/20 09:00
PMCR- 2008/02/03
CRDT- 2007/03/23 09:00
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
PHST- 2008/02/03 00:00 [pmc-release]
AID - 10.1089/neu.2006.0142 [doi]
PST - ppublish
SO  - J Neurotrauma. 2007 Feb;24(2):391-410. doi: 10.1089/neu.2006.0142.