PMID- 17376200
OWN - NLM
STAT- MEDLINE
DCOM- 20070706
LR  - 20211203
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 148
IP  - 3
DP  - 2007 Jun
TI  - Low molecular weight proteins of outer membrane of Salmonella typhimurium are 
      immunogenic in Salmonella induced reactive arthritis revealed by proteomics.
PG  - 486-93
AB  - In patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy 
      (uSpA), synovial fluid mononuclear cells (SFMC) show proliferation to bacterial 
      antigens that trigger ReA, i.e. Chlamydia, Yersinia, Campylobactor, Shigella and 
      Salmonella species. We have shown previously that SFMC proliferate significantly 
      to outer membrane proteins of S typhimurium in Salmonella induced ReA. In the 
      present study we characterized the immunoreactive fractions of outer membrane 
      protein (Omp) of S typhimurium in Salmonella induced ReA. Omp of Salmonella was 
      isolated and fractionated by continuous elution sodium dodecyl 
      sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using Prep-Cell into eight 
      Omp fractions based on molecular weight. Twenty-three patients with ReA were 
      screened for the bacterial trigger using the SFMC proliferative response to crude 
      lysates of Y enterocolitica, S flexneri, C jejuni and S typhimurium using 
      thymidine uptake assay. SFMC from patients with salmonella induced ReA were 
      tested against eight fractions. Seven of 23 patients with ReA had S 
      typhimurium-induced ReA. Of these seven patients, five patients SFMC had a 
      significant stimulation index (SI) against < 22, 22-26, 25-35 and 28-40 kDa 
      fractions of Omp. These fractions were analysed by SDS-PAGE and matrix-assisted 
      laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, which 
      revealed 10 proteins. These proteins were 37 kDa OmpA, 33 kDa TsX, 28 kDa 
      putative Omp, 28 kDa Vac J, 39 kDa OmpD, 18 kDa OmpX, 23 kDa OmpW, 43 kDa OmpS1 
      and 19 kDa peptidoglycan-associated lipoprotein. In conclusion, for the first 
      time we have identified some low molecular weight proteins in the Omps of 
      Salmonella which are T cells immunoreactive in patients with salmonella induced 
      ReA/uSpA.
FAU - Singh, R
AU  - Singh R
AD  - Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical 
      Sciences, Lucknow, India.
FAU - Shasany, A K
AU  - Shasany AK
FAU - Aggarwal, A
AU  - Aggarwal A
FAU - Sinha, S
AU  - Sinha S
FAU - Sisodia, B S
AU  - Sisodia BS
FAU - Khanuja, S P S
AU  - Khanuja SP
FAU - Misra, R
AU  - Misra R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070322
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Immunodominant Epitopes)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Bacterial/chemistry/*immunology
MH  - Arthritis, Reactive/*immunology
MH  - Bacterial Outer Membrane Proteins/chemistry/*immunology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - Immunodominant Epitopes/analysis
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - Prohibitins
MH  - Proteomics
MH  - Salmonella Infections/*immunology
MH  - Salmonella typhimurium/*immunology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Synovial Fluid/immunology
PMC - PMC1941924
EDAT- 2007/03/23 09:00
MHDA- 2007/07/07 09:00
PMCR- 2008/06/01
CRDT- 2007/03/23 09:00
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/07/07 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
PHST- 2008/06/01 00:00 [pmc-release]
AID - CEI3362 [pii]
AID - 10.1111/j.1365-2249.2007.03362.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2007 Jun;148(3):486-93. doi: 10.1111/j.1365-2249.2007.03362.x. 
      Epub 2007 Mar 22.