PMID- 17376308
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20070322
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 120
IP  - 5
DP  - 2007 Mar 5
TI  - Study of the SH3-domain GRB2-like 2 gene expression in laryngeal carcinoma.
PG  - 385-8
AB  - BACKGROUND: Laryngeal carcinoma is a common malignant tumor of the upper 
      respiratory tract, and in 95% of cases the tumor is laryngeal squamous cell 
      carcinoma (LSCC). The abnormity of SH3-domain GRB2-like 2 (SH3GL2) gene was found 
      in LSCC. In order to clarify the relationship between SH3GL2 gene and LSCC, we 
      evaluated the expression of the SH3GL2 gene in LSCC. METHOD: Real-time PCR, 
      immunohistochemistry and Western blotting were used to detect the mRNA and 
      protein expression and find the various rules of SH3GL2 gene in LSCC. RESULTS: 
      The result of real-time PCR showed that the expression level of SH3GL2 mRNA in 
      LSCC tissue was apparently down-regulated; immunohistochemical analysis showed 
      that SH3GL2 protein was mainly located in cytoplasm, the rate of positive cells 
      and SH3GL2 protein expression level were fluctuated with the pathological 
      classification of LSCC; the result of Western blotting showed that SH3GL2 protein 
      was down-regulated significantly in LSCC samples, especially in metastatic lymph 
      nodes. CONCLUSIONS: These results suggest that SH3GL2 is a LSCC related gene and 
      its expression level is fluctuated with the pathological classification which 
      indicate that SH3GL2 participates in the development and progression of LSCC. And 
      it may be considered as a novel tumor marker to find both a new anti-oncogene and 
      relative factors of invasion and metastasis of laryngeal carcinoma.
FAU - Shang, Chao
AU  - Shang C
AD  - Department of Medical Genetics, China Medical University, Shenyang 110001, China.
FAU - Fu, Wei-neng
AU  - Fu WN
FAU - Guo, Yan
AU  - Guo Y
FAU - Huang, Dai-fa
AU  - Huang DF
FAU - Sun, Kai-lai
AU  - Sun KL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (SH3GL2 protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/analysis/*genetics
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/chemistry/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/chemistry/*genetics
MH  - Polymerase Chain Reaction
MH  - src Homology Domains
EDAT- 2007/03/23 09:00
MHDA- 2007/04/25 09:00
CRDT- 2007/03/23 09:00
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 2007 Mar 5;120(5):385-8.