PMID- 17376612
OWN - NLM
STAT- MEDLINE
DCOM- 20070912
LR  - 20220321
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 123
IP  - 1-3
DP  - 2007 Jul 20
TI  - Assessment of the efficacy of commercial porcine reproductive and respiratory 
      syndrome virus (PRRSV) vaccines based on measurement of serologic response, 
      frequency of gamma-IFN-producing cells and virological parameters of protection 
      upon challenge.
PG  - 69-85
AB  - The efficacy of two different types of commercial vaccines against PRRSV 
      (Euro-type) was evaluated based on clinical parameters upon challenge as well as 
      post-challenge virological profiles (viremia and viral load in tissues upon 
      necropsy, measured in both cases by quantitative real time PCR). In an attempt to 
      establish correlates of protective immunity, two commonly proposed parameters 
      predictive of immunity were measured: (1) serologic responses (ELISA and 
      neutralizing antibodies), (2) frequency of gamma interferon-producing cells in 
      peripheral blood mononuclear cell fraction. The vaccines compared consisted of 
      two commercially available products that are regularly marketed in Spain: one 
      modified live virus and one killed vaccine. The efficacy assay was carried out by 
      vaccinating twice 3 weeks apart groups of 5 and-a-half month-old female swine and 
      then challenging them with a European type 1 PRRSV strain (Lelystad). The results 
      obtained indicate that the modified live virus vaccine was the only type of 
      vaccine capable of establishing protective immunity, as measured by viral load in 
      blood and tissues. The killed vaccine, in spite of this product evoking a 
      spontaneous interferon-gamma response and post-challenge titers of 
      virus-neutralizing antibody, evoked no measurable protective immunity. In the 
      case of the modified live vaccine, the protection exhibited did not appear to be 
      based on humoral but rather on cell-mediated immunity.
FAU - Zuckermann, Federico A
AU  - Zuckermann FA
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, USA.
FAU - Garcia, Esther Alvarez
AU  - Garcia EA
FAU - Luque, Ivan Diaz
AU  - Luque ID
FAU - Christopher-Hennings, Jane
AU  - Christopher-Hennings J
FAU - Doster, Alan
AU  - Doster A
FAU - Brito, Monica
AU  - Brito M
FAU - Osorio, Fernando
AU  - Osorio F
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070216
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Chemistry, Pharmaceutical
MH  - Female
MH  - Interferon-gamma/*biosynthesis/metabolism
MH  - Interleukin-10/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/metabolism/*prevention 
      & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Serologic Tests
MH  - Specific Pathogen-Free Organisms
MH  - Swine
MH  - Viral Vaccines/*immunology
EDAT- 2007/03/23 09:00
MHDA- 2007/09/13 09:00
CRDT- 2007/03/23 09:00
PHST- 2006/10/02 00:00 [received]
PHST- 2007/01/27 00:00 [revised]
PHST- 2007/02/09 00:00 [accepted]
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/09/13 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
AID - S0378-1135(07)00079-X [pii]
AID - 10.1016/j.vetmic.2007.02.009 [doi]
PST - ppublish
SO  - Vet Microbiol. 2007 Jul 20;123(1-3):69-85. doi: 10.1016/j.vetmic.2007.02.009. 
      Epub 2007 Feb 16.