PMID- 17376739
OWN - NLM
STAT- MEDLINE
DCOM- 20070920
LR  - 20070420
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 39
IP  - 1
DP  - 2007 May
TI  - Direct detection of human herpesvirus 6 DNA in serum by the loop-mediated 
      isothermal amplification method.
PG  - 22-6
AB  - BACKGROUND: A more rapid and easier method is needed for monitoring human 
      herpesvirus 6 (HHV-6) infections. The loop-mediated isothermal amplification 
      method (LAMP) can detect viral DNA with high specificity, efficiency, and speed 
      under isothermal conditions. LAMP requires only simple equipment that is 
      available in hospital laboratories. OBJECTIVES: We evaluated LAMP as a means of 
      detecting HHV-6 DNA directly from patients' sera. RESULTS: The sensitivity of the 
      HHV-6 LAMP protocol without heat denaturation was 1000 copies/tube; with heat 
      denaturation 10 copies/tube were detected. Three hundred serum samples from 
      children with fever were analyzed. Using HHV-6 isolation as a definition of HHV-6 
      infection, the sensitivity, specificity, positive predictive value, and negative 
      predictive value of the HHV-6 LAMP method without DNA extraction were 95.5%, 
      95.2%, 94.0%, and 96.4%, respectively. CONCLUSION: Direct detection of HHV-6 DNA 
      in serum with a modified HHV-6 LAMP could be used for rapid diagnosis of exanthem 
      subitum (ES).
FAU - Ihira, Masaru
AU  - Ihira M
AD  - Department of Medical Information Technology, Fujita Health University College, 
      Toyoake, Aichi, Japan.
FAU - Akimoto, Shiho
AU  - Akimoto S
FAU - Miyake, Fumi
AU  - Miyake F
FAU - Fujita, Ayano
AU  - Fujita A
FAU - Sugata, Ken
AU  - Sugata K
FAU - Suga, Sadao
AU  - Suga S
FAU - Ohashi, Masahiro
AU  - Ohashi M
FAU - Nishimura, Naoko
AU  - Nishimura N
FAU - Ozaki, Takao
AU  - Ozaki T
FAU - Asano, Yoshizo
AU  - Asano Y
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070321
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - DNA Primers
MH  - DNA, Viral/*blood
MH  - Exanthema Subitum/blood/*virology
MH  - Female
MH  - Fever/blood/virology
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Infant
MH  - Male
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Sensitivity and Specificity
EDAT- 2007/03/23 09:00
MHDA- 2007/09/21 09:00
CRDT- 2007/03/23 09:00
PHST- 2006/08/29 00:00 [received]
PHST- 2007/02/01 00:00 [revised]
PHST- 2007/02/01 00:00 [accepted]
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/09/21 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
AID - S1386-6532(07)00066-2 [pii]
AID - 10.1016/j.jcv.2007.02.001 [doi]
PST - ppublish
SO  - J Clin Virol. 2007 May;39(1):22-6. doi: 10.1016/j.jcv.2007.02.001. Epub 2007 Mar 
      21.