PMID- 17376882
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20210102
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 5
DP  - 2007 May
TI  - Simultaneous quantification of Epstein-Barr virus, cytomegalovirus, and human 
      herpesvirus 6 DNA in samples from transplant recipients by multiplex real-time 
      PCR assay.
PG  - 1426-32
AB  - We developed a multiplex real-time PCR assay using 6-carboxyfluorescein, 
      6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein, and carbocyanine 5-labeled 
      probes to simultaneously quantify Epstein-Barr virus (EBV), cytomegalovirus 
      (CMV), and human herpesvirus 6 (HHV-6) DNA. When previously tested and stored DNA 
      samples were examined, results of the multiplex real-time PCR assay were as 
      sensitive and specific as those of a single real-time PCR assay. The multiplex 
      assay was used to quantify the EBV, CMV, and HHV-6 DNA in 46 transplant 
      recipients. A total of 303 whole-blood and plasma specimens were collected and 
      analyzed. According to the results of the multiplex assay, the detection rates 
      for viral DNA in whole blood and plasma were 23.8% and 5.9% for EBV, 11.2% and 
      5.3% for CMV, and 12.5% and 2.0% for HHV-6, respectively. All forms of viral DNA 
      were detected more frequently in whole blood than in plasma. During the 
      symptomatic period, EBV DNA was detected in all whole-blood specimens but not in 
      all plasma specimens. Furthermore, the EBV DNA load in whole blood was higher 
      during the symptomatic period than during the asymptomatic period, whereas the 
      EBV DNA load in plasma was similar for both periods. These results demonstrate 
      that whole blood is more suitable for the quantification of EBV DNA in transplant 
      patients. However, a cutoff value with clinical relevance still needs to be 
      determined.
FAU - Wada, Kaoru
AU  - Wada K
AD  - Department of Virology, Nagoya University Graduate School of Medicine, 65 
      Tsuruma-cho, Showa-ku, Nagoya 466-8550, Japan.
FAU - Kubota, Naomi
AU  - Kubota N
FAU - Ito, Yoshinori
AU  - Ito Y
FAU - Yagasaki, Hiroshi
AU  - Yagasaki H
FAU - Kato, Koji
AU  - Kato K
FAU - Yoshikawa, Tetsushi
AU  - Yoshikawa T
FAU - Ono, Yasuyuki
AU  - Ono Y
FAU - Ando, Hisami
AU  - Ando H
FAU - Fujimoto, Yasuhiro
AU  - Fujimoto Y
FAU - Kiuchi, Tetsuya
AU  - Kiuchi T
FAU - Kojima, Seiji
AU  - Kojima S
FAU - Nishiyama, Yukihiro
AU  - Nishiyama Y
FAU - Kimura, Hiroshi
AU  - Kimura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070321
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Viral/blood
MH  - Child
MH  - Child, Preschool
MH  - Cytomegalovirus/genetics/*isolation & purification
MH  - Cytomegalovirus Infections/diagnosis/transmission
MH  - DNA, Viral/*blood
MH  - Epstein-Barr Virus Infections/diagnosis/transmission
MH  - Female
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Herpesvirus 4, Human/genetics/*isolation & purification
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Infant
MH  - Liver Transplantation/*adverse effects
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Roseolovirus Infections/diagnosis/transmission
MH  - Sensitivity and Specificity
MH  - Viral Load
PMC - PMC1865882
EDAT- 2007/03/23 09:00
MHDA- 2007/06/15 09:00
PMCR- 2007/09/01
CRDT- 2007/03/23 09:00
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
PHST- 2007/09/01 00:00 [pmc-release]
AID - JCM.01515-06 [pii]
AID - 1515-06 [pii]
AID - 10.1128/JCM.01515-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 May;45(5):1426-32. doi: 10.1128/JCM.01515-06. Epub 2007 
      Mar 21.