PMID- 17377935
OWN - NLM
STAT- MEDLINE
DCOM- 20070529
LR  - 20151119
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 21
IP  - 8
DP  - 2007
TI  - Detection and sequence determination of a new variant beta-lactoglobulin II from 
      donkey.
PG  - 1438-46
AB  - The sequence determination of a new variant of beta-LG II, detected as a minor 
      component by reversed-phase high-performance liquid chromatography/electrospray 
      ionization mass spectrometry (RP-HPLC/ESI-MS) analysis of the whey fraction from 
      a milk sample taken from an individual donkey belonging to the 'Ragusana' species 
      of eastern Sicily, is reported. Direct RP-HPLC/ESI-MS analysis of the whey 
      fraction from this milk sample allowed the identification of a new variant of 
      beta-LG II, based on the determination of the M(r) of the intact protein. The new 
      protein, with an experimentally determined M(r) of 18311 Da, was detected as a 
      minor component in the whey fraction investigated. Matrix-assisted laser 
      desorption/ionization time-of-flight (MALDI-TOF)MS and RP-HPLC/ESI-MS/MS analyses 
      of the tryptic digest of the new protein demonstrate that it presents two amino 
      acid substitutions with respect to the sequence of beta-LG II A, namely a 
      substitution Pro-->Cys at position 110, and a substitution Asp-->Gly at position 
      162. The disulfide bonds between the four cysteines, not directly determined in 
      donkey's and horse's beta-LG II, were shown to occur between Cys(106)-Cys(120) 
      and Cys(66)-Cys(161), as in other mammalian beta-LGs. The new beta-LG II variant 
      from donkey was named D.
CI  - Copyright (c) 2007 John Wiley & Sons, Ltd.
FAU - Cunsolo, Vincenzo
AU  - Cunsolo V
AD  - Dipartimento di Scienze Chimiche, Universita degli Studi di Catania, Viale A. 
      Doria 6, I-95125 Catania, Italy.
FAU - Costa, Alessia
AU  - Costa A
FAU - Saletti, Rosaria
AU  - Saletti R
FAU - Muccilli, Vera
AU  - Muccilli V
FAU - Foti, Salvatore
AU  - Foti S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Lactoglobulins)
RN  - 0 (Milk Proteins)
RN  - 0 (Whey Proteins)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Chromatography, High Pressure Liquid
MH  - *Equidae
MH  - Lactoglobulins/analysis/*chemistry
MH  - Milk/*chemistry
MH  - Milk Proteins/chemistry
MH  - Molecular Sequence Data
MH  - Sequence Analysis, Protein
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Tandem Mass Spectrometry
MH  - Trypsin/chemistry
MH  - Whey Proteins
EDAT- 2007/03/23 09:00
MHDA- 2007/05/30 09:00
CRDT- 2007/03/23 09:00
PHST- 2007/03/23 09:00 [pubmed]
PHST- 2007/05/30 09:00 [medline]
PHST- 2007/03/23 09:00 [entrez]
AID - 10.1002/rcm.2978 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2007;21(8):1438-46. doi: 10.1002/rcm.2978.