PMID- 17379376 OWN - NLM STAT- MEDLINE DCOM- 20070813 LR - 20131121 IS - 0300-483X (Print) IS - 0300-483X (Linking) VI - 234 IP - 1-2 DP - 2007 May 5 TI - Identification of differentially expressed genes in the testis of Sprague-Dawley rats treated with di(n-butyl) phthalate. PG - 103-12 AB - The aim of this study was to identify the di(n-butyl) phthalate (DBP)-induced differentially expressed genes (DEGs) using a novel annealing control primer system in the testes of Sprague-Dawley male rats. Animals (4 weeks of age) were administered orally either corn oil only (vehicle control) or DBP (250, 500, or 750 mg/kg/day) for 30 days. Total RNA was isolated from the rat testes and GeneFishing PCR was used to determine the differential gene expression levels. Using this technique, a total of 59 DEG mRNA fragments were observed in the testes treated with DBP 750 mg/kg/day compared to vehicle control. Of these 59 genes, 31 genes were significantly altered after exposing rats to high dose DBP (750 mg/kg/day), and their sequences cloned. Based on the Basic Local Alignment Search Tool (BLAST), 4 expressed sequence tags (EST), 27 cloned genes (Insl3, pgrp, H1SHR, etc.) and 3 genes (LDHA, lactate dehydrogenase A; Spag4, sperm associated antigen 4 and PBR, peripheral-type benzodiazepine receptor) were found to be involved in spermatogenesis and steroidogenesis. In addition, the expression patterns of the steroidogenesis-related genes such as scavenger receptor class B-1 (SR-B1), steroidogenic acute regulated protein (StAR), P450 side chain cleavage (P450scc), CYP17, and CYP19 were further analyzed by RT-PCR. Significant increases in the mRNA levels of steroidogenesis-related genes (PBR, SR-B1, StAR, P450scc, and CYP17) were observed in the high dose DBP-treated rats. However, DBP significantly decreased the CYP19 mRNA levels compared with controls. DBP (750 mg/kg/day) significantly increased the TR-alpha1 and PPARgamma expression in testes, whereas the AR and ERbeta protein levels were significantly reduced in the same group. These data indicate that the steroidogenesis- or spermatogenesis-related genes identified in this study may provide insights into the molecular mechanisms underlying environmental pollutants-mediated male infertility. FAU - Ryu, Ju Young AU - Ryu JY AD - Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, San 30, Jangjun-Dong, Gumjung-Ku, Busan, South Korea. FAU - Lee, Byung Mu AU - Lee BM FAU - Kacew, Sam AU - Kacew S FAU - Kim, Hyung Sik AU - Kim HS LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070217 PL - Ireland TA - Toxicology JT - Toxicology JID - 0361055 RN - 0 (Estrogen Receptor beta) RN - 0 (PPAR alpha) RN - 0 (Plasticizers) RN - 0 (RNA, Messenger) RN - 0 (Receptors, Androgen) RN - 2286E5R2KE (Dibutyl Phthalate) SB - IM MH - Administration, Oral MH - Age Factors MH - Animals MH - Blotting, Western MH - Dibutyl Phthalate/administration & dosage/*toxicity MH - Dose-Response Relationship, Drug MH - Estrogen Receptor beta/genetics/metabolism MH - Gene Expression Profiling/*methods MH - Gene Expression Regulation/drug effects MH - Genes, erbA/drug effects/genetics MH - Male MH - Organ Size/drug effects MH - PPAR alpha/genetics/metabolism MH - Plasticizers/administration & dosage/toxicity MH - RNA, Messenger/genetics/metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, Androgen/genetics/metabolism MH - Reverse Transcriptase Polymerase Chain Reaction MH - Spermatogenesis/drug effects/genetics MH - Testis/*drug effects/metabolism/pathology EDAT- 2007/03/24 09:00 MHDA- 2007/08/19 09:00 CRDT- 2007/03/24 09:00 PHST- 2006/12/28 00:00 [received] PHST- 2007/02/09 00:00 [revised] PHST- 2007/02/12 00:00 [accepted] PHST- 2007/03/24 09:00 [pubmed] PHST- 2007/08/19 09:00 [medline] PHST- 2007/03/24 09:00 [entrez] AID - S0300-483X(07)00095-9 [pii] AID - 10.1016/j.tox.2007.02.003 [doi] PST - ppublish SO - Toxicology. 2007 May 5;234(1-2):103-12. doi: 10.1016/j.tox.2007.02.003. Epub 2007 Feb 17.