PMID- 17382554
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20171116
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 37
IP  - 1
DP  - 2007 Jan
TI  - Dendritic cells induced in the presence of GM-CSF and IL-5.
PG  - 35-43
AB  - Dendritic cells (DCs), as an important part of antigen-presenting cells, can 
      efficiently prime naive T cell to induce or regulate immune responses. GM-CSF, 
      combined with other growth factors, was used to induce the differentiation of 
      immature or mature DCs from progenitors. Here, we investigated the effect of 
      IL-5, a Th2 cytokine, on the differentiation and function of mouse DCs induced by 
      GM-CSF in vitro. IL-5 significantly inhibited the differentiation of DCs induced 
      by GM-CSF, but no effects on GM-CSF/IL-4 (GM/4) induced DCs. Compared with the 
      conventional mouse DCs developed in the presence of GM-CSF and IL-4, mouse DCs 
      induced by GM-CSF and IL-5 (refereed as to GM/5 DCs) possessed similar cellular 
      morphology, but they expressed high level of CD11c, but low level of MHC II 
      molecules, CD40 and CD86, which is consistent with the immature DC phenotype. In 
      addition, GM/5 DCs showed significantly lower immunogenicity as indicated by 
      their poor stimulating ability to allogeneic T cells in vitro, decreased 
      expression of pro-inflammatory IL-6 and TNF-alpha, as well as increased 
      expression of TGF-beta, compared with GM/4 DCs. Together, these data suggest that 
      IL-5 could partially inhibit GM-CSF-induced DC differentiation which could be 
      reversed by IL-4 in vitro.
FAU - Yi, Huanfa
AU  - Yi H
AD  - Transplantation Biology Research Division, State Key Laboratory of Biomembrane 
      and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, 
      Beijing, China.
FAU - Zhang, Lianjun
AU  - Zhang L
FAU - Zhen, Yu
AU  - Zhen Y
FAU - He, Xiaoyan
AU  - He X
FAU - Zhao, Yong
AU  - Zhao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070326
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (CD11c Antigen)
RN  - 0 (Interleukin-5)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/drug effects/metabolism
MH  - CD11c Antigen/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Shape/drug effects
MH  - Dendritic Cells/*cytology/*drug effects/immunology/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Interleukin-4/pharmacology
MH  - Interleukin-5/*pharmacology
MH  - Interleukin-6/genetics
MH  - Mice
MH  - Phenotype
MH  - T-Lymphocytes/immunology
MH  - Transforming Growth Factor beta/genetics
MH  - Tumor Necrosis Factor-alpha/genetics
EDAT- 2007/03/27 09:00
MHDA- 2007/06/19 09:00
CRDT- 2007/03/27 09:00
PHST- 2006/04/12 00:00 [received]
PHST- 2007/01/06 00:00 [revised]
PHST- 2007/02/07 00:00 [accepted]
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - S1043-4666(07)00027-0 [pii]
AID - 10.1016/j.cyto.2007.02.012 [doi]
PST - ppublish
SO  - Cytokine. 2007 Jan;37(1):35-43. doi: 10.1016/j.cyto.2007.02.012. Epub 2007 Mar 
      26.