PMID- 17383132
OWN - NLM
STAT- MEDLINE
DCOM- 20071231
LR  - 20151119
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 173
IP  - 2-3
DP  - 2007 Dec 20
TI  - Prevalence of gamma-hydroxybutyrate (GHB) in serum samples of amphetamine, 
      metamphetamine and ecstasy impaired drivers.
PG  - 112-6
AB  - Two hundred and forty-seven serum samples which have been collected by police 
      during roadside testing and have been found positive for amphetamine, 
      methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA) and/or 3,4-methylenedioxyethamphetamine 
      (MDE) were analyzed for gamma-hydroxybutyrate (GHB). Serum samples were spiked 
      with deuterated GHB as internal standard and acetonitrile was added to achieve 
      dilution and protein precipitation. Samples were analyzed with a LC-MS/MS system 
      operated in the multiple reaction monitoring mode (MRM) using a TurboIonSpray 
      source. Chromatographic separation was achieved using a Synergi Polar RP column 
      applying a gradient elution with a runtime of 15 min. To differentiate between 
      endogenous and exogenously administered GHB a cut-off concentration of 10 
      microg/mL was applied. Five samples exceeded this concentration and were found 
      positive for GHB. These samples were only found positive for amphetamine but no 
      other amphetamine derivatives were detected, while in three samples THC and in 
      one sample cocaine, benzoylecgonine and ethanol were found.
FAU - Dresen, S
AU  - Dresen S
AD  - Institute of Forensic Medicine, Forensic Toxicology, University Hospital, 
      Albertstr. 9, D-79104 Freiburg, Germany.
FAU - Kempf, J
AU  - Kempf J
FAU - Weinmann, W
AU  - Weinmann W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070323
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Adjuvants, Anesthesia)
RN  - 0 (Amphetamines)
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Dopamine Uptake Inhibitors)
RN  - 3K9958V90M (Ethanol)
RN  - 5353I8I6YS (benzoylecgonine)
RN  - 7G33012534 (Sodium Oxybate)
RN  - I5Y540LHVR (Cocaine)
SB  - IM
MH  - Adjuvants, Anesthesia/*blood
MH  - Adolescent
MH  - Adult
MH  - Amphetamine-Related Disorders/*diagnosis
MH  - Amphetamines/blood
MH  - Automobile Driving/*legislation & jurisprudence
MH  - Central Nervous System Depressants/blood
MH  - Cocaine/analogs & derivatives/blood
MH  - Dopamine Uptake Inhibitors/blood
MH  - Ethanol/blood
MH  - Female
MH  - Forensic Toxicology
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Male
MH  - Sodium Oxybate/*blood
MH  - Substance Abuse Detection
EDAT- 2007/03/27 09:00
MHDA- 2008/01/01 09:00
CRDT- 2007/03/27 09:00
PHST- 2006/08/25 00:00 [received]
PHST- 2007/02/05 00:00 [revised]
PHST- 2007/02/07 00:00 [accepted]
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2008/01/01 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - S0379-0738(07)00094-1 [pii]
AID - 10.1016/j.forsciint.2007.02.009 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2007 Dec 20;173(2-3):112-6. doi: 
      10.1016/j.forsciint.2007.02.009. Epub 2007 Mar 23.