PMID- 17383200
OWN - NLM
STAT- MEDLINE
DCOM- 20081002
LR  - 20070719
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 23
IP  - 3
DP  - 2007 Sep
TI  - Identification and characterization of a myeloid differentiation factor 88 
      (MyD88) cDNA from Zhikong scallop Chlamys farreri.
PG  - 614-23
AB  - Myeloid differentiation factor 88 (MyD88) is a universal and essential adapter 
      for the TLR/IL-1R family. In this report, the first mollusk Myd88 ortholog (named 
      as CfMyd88) was cloned from Zhikong scallop (Chlamys farreri). The full-length 
      cDNA of CfMyd88 was of 1554 bp, including a 5'-terminal untranslated region (UTR) 
      of 427 bp, a polyA tail, and an open reading frame (ORF) of 1104 bp encoding a 
      polypeptide of 367 amino acids containing the typical TLR and IL-1R-related (TIR) 
      domain and death domain (DD). Homology analysis revealed that the predicted amino 
      acid sequence of CfMyd88 was homologous to a variety of previously identified 
      Myd88s with more than 30% identity. The temporal expressions of CfMyd88 mRNA in 
      the mixed primary cultured haemocytes stimulated by lipopolysaccharide (LPS) and 
      peptidoglycans (PGN) were measured by real-time RT-PCR system. The mRNA 
      expression of CfMyd88 decreased after stimulation with both LPS and PGN, and the 
      lowest level was about 1/3 times (at 6 h) and 1/10 times (at 9 h) to that in the 
      control group, respectively. The expression then recovered and was upregulated to 
      two-fold at 9 h after LPS stimulation or to the original level at 12 h after PGN 
      stimulation. The results suggest that the MyD88-dependent signaling pathway 
      exists in scallop and was involved in the defense system.
FAU - Qiu, Limei
AU  - Qiu L
AD  - Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 
      266071, China.
FAU - Song, Linsheng
AU  - Song L
FAU - Yu, Yundong
AU  - Yu Y
FAU - Xu, Wei
AU  - Xu W
FAU - Ni, Duojiao
AU  - Ni D
FAU - Zhang, Qingchun
AU  - Zhang Q
LA  - eng
SI  - GENBANK/DQ249918
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070123
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (DNA, Complementary)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Peptidoglycan)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA, Complementary/*genetics
MH  - *Gene Expression Regulation/drug effects
MH  - Lipopolysaccharides/pharmacology
MH  - Molecular Sequence Data
MH  - Myeloid Differentiation Factor 88/chemistry/*genetics
MH  - Pectinidae/*genetics/*metabolism
MH  - Peptidoglycan/pharmacology
MH  - Sequence Alignment/veterinary
MH  - Sequence Homology, Amino Acid
EDAT- 2007/03/27 09:00
MHDA- 2008/10/03 09:00
CRDT- 2007/03/27 09:00
PHST- 2006/10/13 00:00 [received]
PHST- 2007/01/05 00:00 [revised]
PHST- 2007/01/11 00:00 [accepted]
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2008/10/03 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - S1050-4648(07)00019-8 [pii]
AID - 10.1016/j.fsi.2007.01.012 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2007 Sep;23(3):614-23. doi: 10.1016/j.fsi.2007.01.012. 
      Epub 2007 Jan 23.