PMID- 17383915
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20081121
IS  - 1095-6433 (Print)
IS  - 1095-6433 (Linking)
VI  - 147
IP  - 4
DP  - 2007 Aug
TI  - Cold acclimation and lipid composition in the earthworm Dendrobaena octaedra.
PG  - 911-9
AB  - We have investigated the lipid chemistry during cold acclimation in the freeze 
      tolerant earthworm Dendrobaena octaedra. The dominant phospholipid fatty acids 
      (PLFA) of D. octaedra were 20:4, 20:5 and 20:1 (50% of total PLFA) followed by 
      18:0, 18:1 and 18:2omega6,9 (25% of total PLFA). The ability to tolerate freezing 
      in this species was acquired after acclimation at low temperature for 2-4 weeks. 
      During this period one particular membrane PLFA, 18:2omega6,9, increased 
      significantly and there was a good correlation between the proportion of this 
      PLFA and the survival of freezing. The composition of neutral lipid fatty acids 
      (NLFA), most likely representing storage lipids (triacylglycerides), also changed 
      during cold acclimation so that the overall degree of unsaturation increased. 
      Using a common-garden experiment approach, we compared lipid composition of three 
      genetically different populations (Denmark, Finland and Greenland) that differed 
      in their freeze tolerance. Inter-populational differences and differences due to 
      cold acclimation in overall fatty acid composition were evident in both PLFAs and 
      NLFAs. Specifically, the PLFAs, 20:4 and 20:5, were considerably more represented 
      in worms from Greenland, and this contributed to a higher UI of PLFAs in this 
      population.
FAU - Holmstrup, Martin
AU  - Holmstrup M
AD  - Department of Terrestrial Ecology, National Environmental Research Institute, 
      University of Aarhus, P.O. Box 314, Vejlsovej 25, DK-8600 Silkeborg, Denmark. 
      martin.holmstrup@dmu.dk
FAU - Sorensen, Louise I
AU  - Sorensen LI
FAU - Bindesbol, Anne-Mette
AU  - Bindesbol AM
FAU - Hedlund, Katarina
AU  - Hedlund K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070224
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
RN  - 0 (Phospholipids)
SB  - IM
MH  - *Acclimatization
MH  - Animals
MH  - *Cold Temperature
MH  - Fatty Acids/analysis
MH  - Lipids/*analysis
MH  - Oligochaeta/*chemistry
MH  - Phospholipids/analysis
MH  - Principal Component Analysis
MH  - Time Factors
EDAT- 2007/03/27 09:00
MHDA- 2007/08/02 09:00
CRDT- 2007/03/27 09:00
PHST- 2006/12/01 00:00 [received]
PHST- 2007/02/09 00:00 [revised]
PHST- 2007/02/16 00:00 [accepted]
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - S1095-6433(07)00870-7 [pii]
AID - 10.1016/j.cbpa.2007.02.019 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2007 Aug;147(4):911-9. doi: 
      10.1016/j.cbpa.2007.02.019. Epub 2007 Feb 24.