PMID- 17384204
OWN - NLM
STAT- MEDLINE
DCOM- 20070502
LR  - 20220316
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 9
IP  - 2
DP  - 2007 Apr
TI  - Guidance for fluorescence in situ hybridization testing in hematologic disorders.
PG  - 134-43
AB  - Fluorescence in situ hybridization (FISH) provides an important adjunct to 
      conventional cytogenetics and molecular studies in the evaluation of chromosome 
      abnormalities associated with hematologic malignancies. FISH employs DNA probes 
      and methods that are generally not Food and Drug Administration-approved, and 
      therefore, their use as analyte-specific reagents involves unique pre- and 
      postanalytical requirements. We provide an overview of the technical parameters 
      influencing a reliable FISH result and encourage laboratories to adopt specific 
      procedures and policies in implementing metaphase and interphase FISH testing. A 
      rigorous technologist training program relative to specific types of probes is 
      detailed, as well as guidance for consistent interpretation of findings, 
      including typical and atypical abnormal results. Details are provided on commonly 
      used dual-fusion, extra signal, and break-apart probes, correct FISH nomenclature 
      in the reporting of results, and the use of FISH in relation to other laboratory 
      testing in the ongoing monitoring of disease. This article provides laboratory 
      directors detailed guidance to be used in conjunction with existing regulations 
      to successfully implement a FISH testing program or to assess current practices, 
      allowing for optimal clinical testing for patient care.
FAU - Wolff, Daynna J
AU  - Wolff DJ
AD  - Department of Pathology and Laboratory Medicine, Medical University of South 
      Carolina, Charleston, South Carolina, USA.
FAU - Bagg, Adam
AU  - Bagg A
FAU - Cooley, Linda D
AU  - Cooley LD
FAU - Dewald, Gordon W
AU  - Dewald GW
FAU - Hirsch, Betsy A
AU  - Hirsch BA
FAU - Jacky, Peter B
AU  - Jacky PB
FAU - Rao, Kathleen W
AU  - Rao KW
FAU - Rao, P Nagesh
AU  - Rao PN
CN  - Association for Molecular Pathology Clinical Practice Committee
CN  - American College of Medical Genetics Laboratory Quality Assurance Committee
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (Molecular Probes)
SB  - IM
MH  - Cell Nucleus/metabolism
MH  - Chromosome Banding
MH  - *Genetic Testing
MH  - Hematologic Diseases/*diagnosis
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Molecular Probes
PMC - PMC1867444
EDAT- 2007/03/27 09:00
MHDA- 2007/05/03 09:00
PMCR- 2008/04/01
CRDT- 2007/03/27 09:00
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/05/03 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
PHST- 2008/04/01 00:00 [pmc-release]
AID - S1525-1578(10)60373-X [pii]
AID - 06-0128 [pii]
AID - 10.2353/jmoldx.2007.060128 [doi]
PST - ppublish
SO  - J Mol Diagn. 2007 Apr;9(2):134-43. doi: 10.2353/jmoldx.2007.060128.