PMID- 17384262
OWN - NLM
STAT- MEDLINE
DCOM- 20070426
LR  - 20070326
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1090
DP  - 2006 Dec
TI  - HGF/SF regulates expression of apoptotic genes in MCF-10A human mammary 
      epithelial cells.
PG  - 188-202
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) induces scattering, 
      morphogenesis, and survival of epithelial cells through activation of the MET 
      tyrosine kinase receptor. HGF/SF and MET are involved in normal development and 
      tumor progression of many tissues and organs, including the mammary gland. In 
      order to find target genes of HGF/SF involved in its survival function, we used 
      an oligonucleotide microarray representing 1,920 genes known to be involved in 
      apoptosis, transcriptional regulation, and signal transduction. MCF-10A human 
      mammary epithelial cells were grown in the absence of serum and treated or not 
      with HGF/SF for 2 h. Total RNA was reverse-transcribed to cDNA in the presence of 
      fluorescent Cy3-dUTP or Cy5-dUTP to generate fluorescently labeled cDNA probes. 
      Microarrays were performed and the ratios of Cy5/Cy3 fluorescence were 
      determined. The expression of three apoptotic genes was modified by HGF/SF, with 
      A20 being upregulated, and DAXX and SMAC being downregulated. These changes of 
      expression were confirmed by real-time quantitative PCR. According to 
      current-knowledge, A20 is antiapoptotic and SMAC is proapoptotic, while a pro- or 
      antiapoptotic function of DAXX is controversial. The fact that HGF/SF upregulates 
      an antiapoptotic gene (A20) and downregulates a proapoptotic gene (SMAC) is in 
      agreement with its survival effect in MCF-10A cells. This study identified novel 
      apoptotic genes regulated by HGF/SF, which can contribute to its survival effect.
FAU - Leroy, Catherine
AU  - Leroy C
AD  - CNRS UMR 8161 Institut de Biologie de Lille, Institut Pasteur de Lille, B.P. 447, 
      59021 Lille Cedex, France.
FAU - Deheuninck, Julien
AU  - Deheuninck J
FAU - Reveneau, Sylvie
AU  - Reveneau S
FAU - Foveau, Benedicte
AU  - Foveau B
FAU - Ji, Zongling
AU  - Ji Z
FAU - Villenet, Celine
AU  - Villenet C
FAU - Quief, Sabine
AU  - Quief S
FAU - Tulasne, David
AU  - Tulasne D
FAU - Kerckaert, Jean-Pierre
AU  - Kerckaert JP
FAU - Fafeur, Veronique
AU  - Fafeur V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (DNA Primers)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Apoptosis/*genetics
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Line
MH  - DNA Primers
MH  - Epithelial Cells/metabolism
MH  - Gene Expression Regulation/*physiology
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Mammary Glands, Human/cytology/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/03/27 09:00
MHDA- 2007/04/27 09:00
CRDT- 2007/03/27 09:00
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/04/27 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - 1090/1/188 [pii]
AID - 10.1196/annals.1378.021 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2006 Dec;1090:188-202. doi: 10.1196/annals.1378.021.