PMID- 17384307
OWN - NLM
STAT- MEDLINE
DCOM- 20071026
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 73
IP  - 10
DP  - 2007 May
TI  - High-frequency phage-mediated gene transfer among Escherichia coli cells, 
      determined at the single-cell level.
PG  - 3291-9
AB  - Recent whole-genome analysis suggests that lateral gene transfer by 
      bacteriophages has contributed significantly to the genetic diversity of 
      bacteria. To accurately determine the frequency of phage-mediated gene transfer, 
      we employed cycling primed in situ amplification-fluorescent in situ 
      hybridization (CPRINS-FISH) and investigated the movement of the ampicillin 
      resistance gene among Escherichia coli cells mediated by phage at the single-cell 
      level. Phages P1 and T4 and the newly isolated E. coli phage EC10 were used as 
      vectors. The transduction frequencies determined by conventional plating were 
      3x10(-8) to 2x10(-6), 1x10(-8) to 4x10(-8), and <4x10(-9) to 4x10(-8) per PFU for 
      phages P1, T4, and EC10, respectively. The frequencies of DNA transfer determined 
      by CPRINS-FISH were 7x10(-4) to 1x10(-3), 9x10(-4) to 3x10(-3), and 5x10(-4) to 
      4x10(-3) for phages P1, T4, and EC10, respectively. Direct viable counting 
      combined with CPRINS-FISH revealed that more than 20% of the cells carrying the 
      transferred gene retained their viabilities. These results revealed that the 
      difference in the number of viable cells carrying the transferred gene and the 
      number of cells capable of growth on the selective medium was 3 to 4 orders of 
      magnitude, indicating that phage-mediated exchange of DNA sequences among 
      bacteria occurs with unexpectedly high frequency.
FAU - Kenzaka, Takehiko
AU  - Kenzaka T
AD  - Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, 
      Osaka 565-0871, Japan.
FAU - Tani, Katsuji
AU  - Tani K
FAU - Sakotani, Akiko
AU  - Sakotani A
FAU - Yamaguchi, Nobuyasu
AU  - Yamaguchi N
FAU - Nasu, Masao
AU  - Nasu M
LA  - eng
SI  - GENBANK/AY819041
SI  - GENBANK/AY819042
SI  - GENBANK/AY819043
SI  - GENBANK/AY819044
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070323
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Ampicillin Resistance/genetics
MH  - Bacteriophage P1/genetics/physiology
MH  - Bacteriophage T4/genetics/physiology
MH  - Base Sequence
MH  - Coliphages/*genetics/physiology
MH  - Colony Count, Microbial
MH  - DNA, Viral/chemistry/genetics
MH  - Escherichia coli/*genetics/physiology/*virology
MH  - *Gene Transfer, Horizontal
MH  - Genetic Vectors
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Microbial Viability
MH  - Molecular Sequence Data
MH  - Sequence Analysis
MH  - *Transduction, Genetic
MH  - Viral Plaque Assay
PMC - PMC1907122
EDAT- 2007/03/27 09:00
MHDA- 2007/10/30 09:00
PMCR- 2007/09/01
CRDT- 2007/03/27 09:00
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/10/30 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
PHST- 2007/09/01 00:00 [pmc-release]
AID - AEM.02890-06 [pii]
AID - 2890-06 [pii]
AID - 10.1128/AEM.02890-06 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2007 May;73(10):3291-9. doi: 10.1128/AEM.02890-06. Epub 
      2007 Mar 23.