PMID- 17385213
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20191210
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 109
IP  - 10
DP  - 2007 May 15
TI  - Efficient automated assessment of genetic abnormalities detected by fluorescence 
      in situ hybridization on brush cytology in a Barrett esophagus surveillance 
      population.
PG  - 1980-8
AB  - BACKGROUND: Automated assessment of genetic abnormalities detected by 
      fluorescence in situ hybridization (FISH) in brush cytology specimens from 
      patients with Barrett esophagus (BE) may enhance the clinical applicability of 
      this methodology. The objectives of this study were to validate a novel, 
      automated, proprietary system (CytoVison SPOT AX) for the assessment of FISH 
      abnormalities in BE brush cytology and, subsequently, to use this automated 
      method for screening of a BE surveillance cohort. METHODS: FISH with DNA probes 
      for chromosomes 9, 17, and Y, and for the 9p21 (p16), 17q11.2 (Her2/neu), and 
      17p13.1 (p53) loci was applied on brush cytology specimens from a surveillance 
      cohort of 151 patients with BE. Validation of the automated system was performed 
      by comparison of the automated FISH results with manual scores for the first 60 
      patients. RESULTS: There was 98% concordance between manual and automated FISH 
      analysis with kappa values from 0.49 to 1 for the different probes. The loss of 
      17p13.1 (p53) was observed in only 5% of patients with no dysplasia (ND) and in 
      9% of patients with low-grade dysplasia (LGD) but increased to 46% in patients 
      with high-grade dysplasia (HGD) (P < .005; Fisher exact test). Chromosomes 9 and 
      17 were observed in 6% of patients with ND, in 21% of patients with LGD, and in 
      62% of patients with HGD (P < .05). Ten percent of patients with ND had loss of 
      the Y chromosome, which increased to 27% in patients with HGD (P< .05). The 
      amplification of 17q11.2 (Her2/neu) was detected in 62% of patients with HGD (P < 
      .001). CONCLUSIONS: The current investigation indicated that the CytoVison SPOT 
      AX is an objective, efficient system for the analysis of DNA-FISH on BE brush 
      cytology and is applicable for analyzing large populations of BE patients. In the 
      current study cohort, the loss of 17p13.1 (p53), Y chromosome loss, and polysomy 
      of chromosomes 17 and 9 were correlated with increasing grade of dysplasia in 
      patients with BE.
CI  - (c) 2007 American Cancer Society
FAU - Rygiel, Agnieszka M
AU  - Rygiel AM
AD  - Department of Experimental Internal Medicine, Academic Medical Center, Amsterdam, 
      the Netherlands. a.lukuc@amc.uva.nl
FAU - van Baal, Jantine W P M
AU  - van Baal JW
FAU - Milano, Francesca
AU  - Milano F
FAU - Wang, Kenneth K
AU  - Wang KK
FAU - ten Kate, Febo J
AU  - ten Kate FJ
FAU - Fockens, Paul
AU  - Fockens P
FAU - Rosmolen, Wilda D
AU  - Rosmolen WD
FAU - Bergman, Jacques J G H M
AU  - Bergman JJ
FAU - Peppelenbosch, Maikel P
AU  - Peppelenbosch MP
FAU - Krishnadath, Kausilia K
AU  - Krishnadath KK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Automation
MH  - Barrett Esophagus/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - *Cytogenetic Analysis
MH  - Cytological Techniques
MH  - Female
MH  - Genes, erbB-2/genetics
MH  - Genes, p16
MH  - Genes, p53/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Male
MH  - Middle Aged
EDAT- 2007/03/27 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/03/27 09:00
PHST- 2007/03/27 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/03/27 09:00 [entrez]
AID - 10.1002/cncr.22643 [doi]
PST - ppublish
SO  - Cancer. 2007 May 15;109(10):1980-8. doi: 10.1002/cncr.22643.