PMID- 17385683
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20070402
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 79
IP  - 5
DP  - 2007 May
TI  - Quantitative detection of herpes simplex virus DNA in the lower respiratory 
      tract.
PG  - 597-604
AB  - Quantitation of herpes simplex virus (HSV) DNA in bronchoalveolar lavage 
      specimens could indicate an infectious role in the lower respiratory tract. The 
      aim of this study was to compare quantitative HSV DNA results from adult 
      bronchoalveolar lavage specimens to clinical outcome. Quantitative real-time PCR 
      assays targeting HSV and other herpes viruses were performed on adult 
      bronchoalveolar lavage specimens obtained from a largely immunocompromised 
      population during a 1-year period. The results were compared to patient 
      characteristics and outcome. HSV DNA was detected in 11 (19%) of 57 
      bronchoalveolar lavage specimens with a mean viral level of 5.6 log genome 
      equivalents/ml (range, 2.9-8.1 log). A threshold of HSV DNA levels equal or 
      higher than 5.0 log (n = 7) was associated with mortality within 28 days 
      following hospital admission (odds ratio [OR], 6.8; 95% confidence interval [CI], 
      1.2-39.2). A threshold level of 5.5 log was associated with mortality within 28 
      days of sampling (OR 8.5; 95% CI 1.2-62.1), only after excluding patients 
      receiving specific antiviral medication. Patients with HSV DNA levels equal or 
      higher than 7.5 log had severe respiratory failure. Viral pneumonia was 
      histologically proven in one patient with 8.0 log at autopsy. No patient with HSV 
      DNA levels below 5.5 log (n = 5) or DNA levels higher than 5.0 log of 
      cytomegalovirus (CMV) (n = 3), Epstein-Barr virus (EBV) (n = 9), varicella-zoster 
      virus (VZV) (n = 1), or human herpesvirus 6 (HHV-6) (n = 0) died within 28 days 
      of hospital admission. We conclude that quantitative detection of HSV DNA in 
      bronchoalveolar lavage fluid is a potential diagnostic tool for detection of 
      relevant viral infection of the lower respiratory tract.
FAU - Gooskens, J
AU  - Gooskens J
AD  - Department of Medical Microbiology, Center of Infectious Diseases, Leiden, 
      University Medical Center, P.O. Box 9600, 2300 RC, Leiden, The Netherlands. 
      j.gooskens@lumc.nl
FAU - Templeton, K E
AU  - Templeton KE
FAU - Claas, E C
AU  - Claas EC
FAU - van Bussel, M J
AU  - van Bussel MJ
FAU - Smit, V T
AU  - Smit VT
FAU - Kroes, A C
AU  - Kroes AC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bronchoalveolar Lavage
MH  - Colony Count, Microbial
MH  - DNA, Viral/*analysis
MH  - Diagnosis, Differential
MH  - Disease Progression
MH  - Female
MH  - Herpes Simplex/*diagnosis
MH  - Humans
MH  - Immunocompromised Host
MH  - Male
MH  - Middle Aged
MH  - Pneumonia, Viral/diagnosis
MH  - Respiratory Tract Infections/*diagnosis
MH  - Simplexvirus/*isolation & purification
EDAT- 2007/03/28 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/03/28 09:00
PHST- 2007/03/28 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/03/28 09:00 [entrez]
AID - 10.1002/jmv.20861 [doi]
PST - ppublish
SO  - J Med Virol. 2007 May;79(5):597-604. doi: 10.1002/jmv.20861.