PMID- 17386407
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20131121
IS  - 1567-5769 (Print)
IS  - 1567-5769 (Linking)
VI  - 7
IP  - 5
DP  - 2007 May
TI  - Involvement of mast cells in the regulation of matrix metalloproteinase-9 and 
      tissue inhibitor of metalloproteases-1 in 
      12-O-tetradecanoylphorbolacetate-induced inflammation in mice.
PG  - 597-603
AB  - In this study, we investigated the involvement of mast cells in the regulation of 
      matrix metalloproteinase-9 (MMP-9) in 12-O-tetradecanoylphorbolacetate 
      (TPA)-induced inflammation, using mast cell-deficient (W/W(v)) mice and control 
      (+/+) mice. Topical application of TPA to the ears induced acute inflammation, 
      accompanied by mast cell degranulation in +/+ mice, which peaked at 6-12 h. There 
      was no significant difference in ear thickness between the groups until 12 h, but 
      the swelling was greater in W/W(v) mice than +/+ mice at 24-36 h. Western blot 
      analysis revealed that TPA-induced marked increases in levels of proMMP-9 and 
      tissue inhibitor of metalloproteinases-1 (TIMP-1), which existed as complexes 
      with proMMP-9. The amount of proMMP-9-TIMP-1 complex was markedly smaller in +/+ 
      mice than W/W(v) mice at 6 and 24 h, but had almost returned to control levels in 
      both groups at 48 h. The free form of proMMP-9 was also slightly less abundant in 
      +/+ mice than W/W(v) mice at 6, 24, and 48 h. Gelatin zymographic analysis 
      revealed that levels of the active species of MMP-9 (approximately 74 and 83 kD), 
      as well as free form of proMMP-9, increased time-dependently after the 
      application of TPA and peaked at 24 h in +/+ mice. The 74-kD band was detected 
      only in +/+ mice at 6 h. Our results therefore suggested that during inflammation 
      degranulation of mast cells results in a reduction of the proMMP-9-TIMP-1 complex 
      levels, together with a fall in the amount of free proMMP-9.
FAU - Iba, Yoshinori
AU  - Iba Y
AD  - Department of Pathophysiological Science, Faculty of Pharmaceutical Sciences, 
      Setsunan University, Hirakata 573-0101, Japan.
FAU - Shirai, Maiko
AU  - Shirai M
FAU - Dei, Chiaki
AU  - Dei C
FAU - Hirata, Takahiro
AU  - Hirata T
FAU - Harada, Chika
AU  - Harada C
FAU - Masukawa, Tohru
AU  - Masukawa T
LA  - eng
PT  - Journal Article
DEP - 20070130
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Degranulation/drug effects
MH  - Ear, External/pathology
MH  - Inflammation/chemically induced/enzymology/*metabolism
MH  - Mast Cells/drug effects/*physiology
MH  - Matrix Metalloproteinase 9/*biosynthesis
MH  - Mice
MH  - *Tetradecanoylphorbol Acetate
MH  - Tissue Inhibitor of Metalloproteinase-1/*biosynthesis
EDAT- 2007/03/28 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/03/28 09:00
PHST- 2006/10/07 00:00 [received]
PHST- 2006/12/27 00:00 [revised]
PHST- 2006/12/29 00:00 [accepted]
PHST- 2007/03/28 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/03/28 09:00 [entrez]
AID - S1567-5769(07)00005-7 [pii]
AID - 10.1016/j.intimp.2006.12.012 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2007 May;7(5):597-603. doi: 10.1016/j.intimp.2006.12.012. 
      Epub 2007 Jan 30.